| 硒、维生素E对自由基损伤培养兔软骨细胞的保护作用 |
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| 引用本文： | 王水利,魏西秦,朱杰,张健.硒、维生素E对自由基损伤培养兔软骨细胞的保护作用[J].西安交通大学学报(医学版),1995(3). |
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| 作者姓名： | 王水利 魏西秦 朱杰 张健 |
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| 作者单位： | 西安医科大学病理学教研室 西安710061 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 采用细胞培养方法,以黄嘌岭-黄嘌岭氧化酶(XXO)系统产生自由基,造成软骨细胞的自由基损伤;软骨细胞DNA及蛋白多糖的合成受到明显抑制;膜流动性降低;电镜下观察到细胞表面有大小形态各异的囊泡状突起,胞内可见线粒体肿胀或呈固缩样改变、溶酶体增多等超微病理损害。向培养基中提前1d加入硒及维生素E能对抗自由基所致的氧化性损伤。
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| 关 键 词： | 软骨细胞 自由基 硒 维生素E |
THE PROTECTIVE EFFECT OF SELENIUM OR VITAMINE ON CULTURED RABBIT ARTICULAR CHONDROCYTES INJURED BY OXYGEN FREE-RADICALS |
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| Wang Shuili,Wei Xiqin,Zbu Jie,et al.THE PROTECTIVE EFFECT OF SELENIUM OR VITAMINE ON CULTURED RABBIT ARTICULAR CHONDROCYTES INJURED BY OXYGEN FREE-RADICALS[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1995(3). |
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| Authors: | Wang Shuili Wei Xiqin Zbu Jie |
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| Institution: | Department of Pathology |
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| Abstract: | In this paper, using the method of the rabbit articular chondrocytes (RACs) in the monolayer culture in vitro, we studied the effect of superoxide free-radical generated by the xanthine-xanthine oxidase (XXO) system on RACs. The results showed that oxygen free-radicals inhibited the synthesis of RACs' DNA and proteoglycan, decreased membrane fluidity. The chondrocytes grew slowly and were not confluent under microscope af-ter six days. The changes of cellular ultrastructural damage under TEM and SEM were observed clearly. Selenium or vitamin E added into the medium 24 hours before adding the XXO system was able to protect RACs from being injured by free radicals. These results suggest that selenium or vitamin E may play an important role in retarding the process of cartilage disease. |
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| Keywords: | chondrocyte free-radical selenium vitamin E |
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