角膜实质层胶原纤维三维交叉网状结构的进一步探讨兼论弹性纤维 Ⅰ.兔角膜的LM、TEM及SLM观察

本文应用常规及自行设计的标本制备法对8个正常兔角膜实质层纤维成分分别做了LM、TEM及SEM观察。结果表明:①胶原纤维排列成三维交叉网状結构,后者在约500μm范围内呈板状,称微板,微板相互吻合成微板网;②在LM下所见的厚约4—9μm的“胶原纤維”或“板层”,实际是微板网的垂直切面,⑧文献中的板层结构TEM照片实为其网状结构的一部分,④在胶原纤维之间有少量分支交错的细小纤维,可能是弹性纤维。

Further Study on the 3-dimensional Criss-crossed Reticular Structure of the Collagenous Fibers of the Corneal Stroma with Special Reference to the Elastic Fibers Ⅰ. The LM,TEM and SEM Observations on the Rabbit Corneas

Using both the standard preparations and methods we designed, the corneas obtained from 8 rabbits were observed by means of the LM, TEM and SEM. The results are as follows: 1)The collagenous fibers are arranged in a crass-crossed network of three dimentions. The network in an area of less than 500 μm is like a small board called a microboard. All microboards anastomose each other, forming a microboard network (MBN); 2)The so-callcd collagenous fibers or lamellae about 4—9 μm thick under the LM are really the vertical sections of the MBN; 3)The transmission electronmicrograph of the lamellar structure in the literature is a part of the network; 4)Between the collagenous fibers there are a small number of very fine fibers with branches and criss-crossed arrangement, which might be elastic fibers.