| 一种高敏感度的辣根过氧化物酶新底物用于ELISA检测乙型肝炎表面抗原 |
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| 引用本文： | 沈琼,彭宣宪,方亮.一种高敏感度的辣根过氧化物酶新底物用于ELISA检测乙型肝炎表面抗原[J].西安交通大学学报(医学版),1987(3). |
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| 作者姓名： | 沈琼 彭宣宪 方亮 |
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| 作者单位： | 西安医科大学病毒学研究室，西安医科大学病毒学研究室，西安医科大学病毒学研究室 |
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| 摘 要： | 本文介绍3.3'、5.5'—四甲基联苯胺(TMB)作为辣根过氧化物酶(HRP)的底物应用于ELISA检测乙型肝炎表面抗原(HB_sAg)。TMB敏感性高、反应产色鲜明、本底反应低、终止后稳定,用于检测HB_sAg的ELISA系统,使敏感性比目前最常用的底物邻苯二胺(OPD)提高了8～16倍。TMB是一个比较理想的HRP底物,用于检测HB_sAg的ELISA系统可以提高阳性检出率,有一定的实用价位。
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A NEW SUBSTRATE OF HORSE-RADISH PEROXIDASE APPLIED TO INCREASE THE SENSITIVITY OF ELISA FOR DETECTION HEPATITIS B SURFACE ANTIGEN IN SERA |
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| Shen Qiong,et al.A NEW SUBSTRATE OF HORSE-RADISH PEROXIDASE APPLIED TO INCREASE THE SENSITIVITY OF ELISA FOR DETECTION HEPATITIS B SURFACE ANTIGEN IN SERA[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1987(3). |
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| Authors: | Shen Qiong |
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| Institution: | Reaserch Laboratory of Virology |
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| Abstract: | 3,3,'5,5'-tetramethyl-benzidine(TMB) a new substrate of horse-radish peroxidase(HRP) has been used in ELISA for the detection of Hepatitis B surface antigen(HBsAg)in sera from patients with Hepatitis B and health persons. TMB's main advantages arc noncarcinogenie,highly sensitive, and rather stable. The optimum reactive conditions of TMB in this experiment has been determined. TMB as the substrate of HRP could increase the sensitivity of ELISA for detection HB~sAg for 8 to 16 times more than the ordinary subatrate of HRP, O-Phenylenediamine(OPD), and the positive detecting rate of HB_sAg for about17per cent.TMB is a better substrate of HRP at present. |
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| Keywords: | 3 3' 5 5'-tetrameshyl-benzidine ELISA HBAg substrate of HRP |
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