| 大鼠下丘脑培养中β-内啡肽神经元的免疫细胞化学研究 |
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| 引用本文： | 宋天保,姚向民,张芳婷,周劲松,王丽蓉.大鼠下丘脑培养中β-内啡肽神经元的免疫细胞化学研究[J].西安交通大学学报(医学版),1997(3). |
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| 作者姓名： | 宋天保 姚向民 张芳婷 周劲松 王丽蓉 |
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| 作者单位： | 西安组织学与胚胎学教研室!西安710061 |
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| 摘 要： | 应用种经细胞培养、免疫细胞化学和图像分析技术，研究新生大鼠下丘脑β-内啡肽（β-END）神经元的形态及其体外发育规律。β-END神经元多为梭形双极，可见细胞间接触。培养1d,β-END神经元甚少，3～7d其生长迅速，细胞数量、胞体大小、染色强度和突起长度均达最高峰。培养14d，β-END神经元基本维持在7d时的水平。结果提示，下丘脑β-END神经元在体外培养的第1周内迅速发育趋于成熟，并可维持到第14天左右。
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| 关 键 词： | β-内啡肽神经元 下丘脑 细胞培养 免疫细胞化学 新生大鼠 |
IMMUNOCYTOCHEMICAL STUDY ON β-ENDORPHIN NEURONS IN CULTURES OF RAT HYPOTHALAMUS |
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| Song Tianbao, Yao Xiangmin, Zhang Fangting et al.IMMUNOCYTOCHEMICAL STUDY ON β-ENDORPHIN NEURONS IN CULTURES OF RAT HYPOTHALAMUS[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1997(3). |
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| Authors: | Song Tianbao Yao Xiangmin Zhang Fangting |
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| Institution: | Dapartment of Histology and Embryology |
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| Abstract: | The morphology and development of cultured newborn rat hypothalamic β-END neurons were studied by using immunocytochemical tech-nique and image analysis. β-END neurons were gen-erally fusiform and bipolar and intercellular contacts were found between them. A few fyEND neurons were found on in vitro day l. They grew fast during days 3-7 and reached the highest levels of their number, cell body size, staining intensity and pro-cess length on day 7. On day 14, the soma size and process length of β-END neurons were maintained at the level of day 7, but their number and staining in-tensity were decreased. The results indicate that the hypothalamic PEND neurons develop to maturation in the first week of culture and maintain this state up to day 14- |
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| Keywords: | β-END neuron hypothalamus cell culture immunocytochemistry newborn rat |
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