| 用ELISA法检测GSH-PX活性及其在克山病病区儿童中的初步应用 |
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| 引用本文： | 韩.用ELISA法检测GSH-PX活性及其在克山病病区儿童中的初步应用[J].西安交通大学学报(医学版),1989(1). |
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| 作者姓名： | 韩 |
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| 作者单位： | 西安医科大学中西医结合研究所
(韩(王从))，西安医科大学克山病研究室
(谭武红)，西安医科大学中西医结合研究所(耿涛) |
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| 摘 要： | 本文建立了检测血GSH-px 的ELISA 双抗体夹心法。用此方法对克山病病区患儿及健康儿童的血液GSH-px 活力进行了测定。发现病区慢型克山病、潜在型克山病以及健康儿童血GSH-px 活力均明显低于非病区健康儿童(p<0.01);慢型克山病患儿血GSH-px 活力低于病区健康儿童(p<0.05);病区健康儿童血GSH-px 活力与潜在型克山病患儿无差异(p>0.05)。本文对实验方法及结果进行了初步讨论。
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| 关 键 词： | 酶联免疫吸附法 谷胱甘肽过氧化物酶 克山病 |
ELISA IN DETECTION OF BLOOD GSH-PX AND ITS PRELIMINARY APPLICATION IN KESHAN DISEASE |
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| Han Cong,et al.ELISA IN DETECTION OF BLOOD GSH-PX AND ITS PRELIMINARY APPLICATION IN KESHAN DISEASE[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1989(1). |
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| Authors: | Han Cong |
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| Institution: | Institute of Combined Traditional Chinese Western Medicine |
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| Abstract: | An ELISA bi-antibody sandwich for thedetection of human blood GSH-px has beenestablished.GSH-px activities were deter-mined ELISA in children in Keshan diseaseeffected aras and non-effected areas.Theresults showed that the blood GSH-px acti- vities in the children(latent type,chronictype anti healthy children)in the Keshandisease effected areas were lower than thesein the non-effected areas,with a signifi-cant difference(P<0.01),However,theblood GSH-px activities in chronic typechildren were lower than in healthy child-ren in the Keshan disease effected areas(P<0.05). |
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| Keywords: | ELISA GSH-px Keshan disease |
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