DAMAGES OF GYPENOSIDES ON THE ULTRASTRUCTURES OF S-180 SARCOMA IN MOUSE

Gypenosides(GP)wasextractedfromGynostemmapentaphyllam(Thunb.)Markc17.LiteraturereportandourlaboratorystudiedresultsshowedthatGPhadsignificantinhibitionactiontomanykindsoftumorstraint23.InordertorevealthemechanismofantitumorofGP,westudiedtheeffectsofGPontheultrastructureofS--180sarcomacellsinmouse.MATERIALSANDMETHODSKun-iningspeciesmouse,weighing22t2g,eithersex,injecteds.c.0.2ml(contained4X105cells)cellsuspendingsolutionofS-180sarcoma,wasrandomizedintofourgroups:Salinecontrol(n'30…

DAMAGES OF GYPENOSIDES ON THE ULTRASTRUCTURES OF S-180 SARCOMA IN MOUSE

The significant inhibition action of Gypenosides (GP) to many kinds or tumor strain wesreported before. The erfects of GP on the ultrastructures, without any findiys in present litrature,of S-180 sarcoma in mouse were studied through electron microscope in this article. The results indicated that the ultrastructures of.S-180 sarcoma cells were destructed obviously by the administratiouof GP to the tumor-bearing mouse with a Pattern or dose-depeudant, especially to the cellular nuclear. We saw both apoptosis and necrosis in morphologic alterations in tumor cells, suck as a reductionin cellular volume. an increase in cytoplasm, uucleoplasm electron density and condensation of nuclear chromatiu either to periphery or the nuclear membrane or inclumps within the cell, lots of inthe cytoplasm and apoptotic body in some turner cells or some cytoplast breaking into small frag- ments etc.