转录调节因子E26转录因子-1蛋白在胎膜早破中的表达和意义

目的 探讨E26转录因子-1(E26 transformation specific-1, Ets-1)蛋白在胎膜早破中的表达和意义.方法 采用免疫组织化学S-P法在蛋白水平检测75例胎膜早破者(病例组)及70例无其他产科并发症及内外科合并症的足月择期剖宫产者(对照组)胎膜中Ets-1蛋白的表达.结果 ①Ets-1蛋白在胎膜滋养层细胞核及胞浆中均有表达,以胞核表达为主;②Ets-1蛋白在胎膜早破组滋养层中高表达,与对照组比较,有显著性差异(P<0.05);③Ets-1蛋白在未足月胎膜早破组滋养层中高表达,但与足月胎膜早破组比较,无显著性差异(P>0.05).结论 转录调节因子Ets-1在人类胎膜上有表达,且在胎膜早破中高表达,表明Ets-1蛋白可能使胎膜细胞外基质重塑从而引发胎膜早破.本研究可作为预测胎膜早破发生的依据.

Expression and clinical signficance of the transcription factor E26 transformation specific-1 in premature rupture of fetal membranes

Objective To examine the expression and clinical significance of E26 transformation specific-1 in premature rupture of fetal membranes. Methods Fetal membranes from 75 women in the following categories were analyzed for Ets-1 expression: preterm and term premature rupture of fetal membranes; 70 women (control group) with term cesarean sections and without complications. Ets-1 protein was localized with the use of immunohistochemical S-P method. Results Ets-1 protein was expressed in both the nucleus and cytoplasm of trophoblast of human fetal membranes, with more obvious expression in the nucleus. Ets-1 protein's expression was up-regulated in the trophoblast of fetal membranes with premature rupture, which differed significantly from the control group (P<0.05). Ets-1 protein's expression was up-regulated in the trophoblast of fetal membranes with preterm premature rupture, which did not differ significantly from the control group (P>0.05). Conclusion Ets-1 is expressed in human fetal membranes and its expression is up-regulated with premature rupture of fetal membranes, suggesting a role for Ets-1 in extracellular matrix remodeling of the membranes. This study provides an evidence to predict premature rupture of fetal membrances.