克山病病区粮对仔猪与大白鼠组织溶酶体酶活性的影响

本文测定了饲克山病病区粮 (Se 0.041PPm) 与病区粮加硒 (Se 0.25PPm) 两组仔猪的心肌和肝脏及大白鼠肝脏溶酶体组织蛋白酶D,透明质酸酶活性。病区粮仔猪心肌和肝脏组织蛋白酶D和心肌透明质酸酶的活性显著高于加硒组仔猪,但肝脏透明质酸酶的活性增高不显著。病区粮组大白鼠肝脏组织蛋白酶D的活性亦非常显著高于加硒组大白鼠,但透明质酸酶活性增高不显著。实验结果初步表明病区粮组仔猪和大白鼠心肌和肝脏的两种与蛋白质,粘多糖分解代谢有关的溶酶体酶异常。

Effect of the Diet in the Keshan Disease Areas on the Tissue Lysosomal Enzyme Activities in Pigs and Rats

To elucidate the metabolic abnormality of eardiomyopathy and diefary liver necrosis in selenium-deficient pigs and rats, two lysosomal enzyme activities were investigated. Tissues for enzyme assays were taken from the pigs and rats fed with the diet in the Keshan disease areas(Se 0.014PPm, a selenium-deficient diet) and the same diet supplemented with selenium (Se 0.25ppm) in the control group. The activities of lysosomal cathepsin D and hyaluronidase in the myocardium and liver of the pigs and rats were determined. The results of our observation suggest that the specific activities of cathepsin D in the myoeardium and liver significantly higher in the selenium-defieient pigs than in normal pigs. The speficic activity of hyaluronidase is significantly higher in themyocardium, but not in the liver of the selenium-deficient pigs(P0.05). The speficie aetivity of ca thepsin D, but not hyaluronidase, in the liver is also sifnifieantly higher in zhe selenium-deficient rats than in the normal rats(P0.01). The present study suggests that abnormalities in lysosomal enzyme ae tivities are associated with catabolism of protein and mueopolysacchar ide under this seleniumdeficient condition. The changes in activities of cathepsin D and hyaluronidase in relation to the pathological changes of tissue damage are also discussed breifly.