Proteomics Related to Resistance to the Cucumber Powdery Mildew Disease in F2Generation of Cucumber

Cucumber powdery mildew disease caused bySphaerotheca fuliginea is a serious disease of culti-vated cucumber in most cucumber-growing regionsof the world. Understanding resistant mechanismof cucumber against powdery mildew disease is in-creasingly crucial for breeding resistant cultivars toeffectively overcome the disease. In the case, agreat deal of researches on single proteins or en-zymes encoded by the resistant genes have beenperformed to uncover defense mechanism. Howev-er, so far there …

Proteomics Related to Resistance to the Cucumber Powdery Mildew Disease in F_2 Generation of Cucumber

Proteomic approaches using two-dimensional gel electrophoresis (2-DE) were adopted to identify proteins from cucumber leaves differentially expressed in F_2 generation of cucumber. Nearly 500 proteins were reproducibly detected, including 39 specific proteins expressed in F_2 sensitive pool samples and 22 specific proteins expressed in resistance pool samples, 35 proteins were two-fold upper limit and 46 proteins were two-fold lower limit. Furthermore, analysis with MALDI-TOF-MASS showed that one important protein of disease resistance gene (SSP3110) identified in F_2 generation of cucumber associated with defensive reaction. Three proteins (SSP5412, SSP5513, SSP5710) related to respiration or photosynthesis were identified in F_2 resistant pool samples, and 14-3-3 brain protein homolog (SSP1411) was identified in F_2 sensitive pool samples. All those proteins were probably parts of network of resistance or development-related proteins. This study gives new insights into cucumber powdery mildew resistant response in cucumber leaves and demonstrates the power of the proteomic approach in plant biology studies. MS analysis of other protein spots is underway.