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| 他汀类药物对神经细胞和神经胶质细胞ABCA1基因表达的影响 | |
| 引用本文： | 宫惠琳,李扩,张冠军,侯惠莲,董炜疆.他汀类药物对神经细胞和神经胶质细胞ABCA1基因表达的影响[J].西安交通大学学报(医学版),2010,31(5). |
| 作者姓名： | 宫惠琳 李扩 张冠军 侯惠莲 董炜疆 |
| 作者单位： | 1. 西安交通大学医学院,第一附属医院病理科,陕西,西安,710061 2. 西安交通大学医学院,第一附属医院神经外科,陕西,西安,710061 3. 西安交通大学医学院,人体解剖与组织胚胎学系,陕西,西安,710061 |
| 基金项目： | 美国NIH;NHLBI资助项目，国家自然科学基金资助项目，陕西省科技攻关项目 |
| 摘 要： | 目的 观察他汀类药物对神经细胞和神经胶质细胞ATP结合盒式转运体1(ATP-binding cassette transporter 1,ABCA1)基因表达的影响.方法 培养人类神经细胞和神经胶质细胞,分别加入不同浓度的辛伐他汀(simvastatin)和普伐他汀(pravastatin),孵育不同时间后分别提取细胞总RNA和胞质蛋白,RT-PCR观察ABCA1基因的表达,Western blot检测ABCA1蛋白.结果 辛伐他汀和普伐他汀组显著降低了ABCA1基因和蛋白的表达,其中辛伐他汀的作用比普伐他汀明显(辛伐他汀对神经元和神经胶质细胞ABCA1基因表达分别降低95%和75%P<0.05),神经元实验组比神经胶质细胞组更为敏感.结论 他汀类药物可以显著降低神经元和神经胶质细胞ABCA1基因和蛋白的表达,ABCA1的降低有可能影响细胞内胆固醇的代谢,对全面评价他汀类药物对高血脂相关基因的影响及用药安全性提供了一定的实验依据. |
| 关 键 词： | ATP结合盒式转运体1 辛伐他汀 普伐他汀 |
Effect of statins on ABCA1 gene expression in neurons and astrocytes |
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| GONG Hui-lin,LI Kuo,ZHANG Guan-jun,HOU Hui-lian,DONG Wei-jiang.Effect of statins on ABCA1 gene expression in neurons and astrocytes[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2010,31(5). | |
| Authors: | GONG Hui-lin LI Kuo ZHANG Guan-jun HOU Hui-lian DONG Wei-jiang |
| Institution: | GONG Hui-lin1,LI Kuo2,ZHANG Guan-jun1,HOU Hui-lian1,DONG Wei-jiang3(1.Department of Pathology,the First Affiliated Hospital,2.Department of Neurosurgery,3.Department of Anatomy and Histology & Embryology,Medical School of Xi'an Jiaotong University,Xi'an 710061,China) |
| Abstract: | Objective To observe the effects of statins on ATP-binding cassette transporter 1(ABCA1) gene expression in neurons and astrocytes.Methods Primary human astrocytes and neuron cell line HCN-2 were incubated with simvastatin and pravastatin at different concentrations and under different conditions.Using RNA isolated from the cultured cells,we performed quantitative PCR to measure the ABCA1 gene expression in astrocytes and neurons.The protein expression of ABCA1 was measured by Western blot.Results The two s... |
| Keywords: | ATP-binding cassette transporter 1(ABCA1) simvastatin pravastatin |
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