对氧磷-酶1192位Gln-Arg基因多态性与冠心病的相关性

目的确定对氧磷酶1(PON1)基因192位Gln-Arg多态性与陕西地区汉族冠心病(CHD)患者发病之间的关系。方法对动脉造影确诊的222例冠心病患者和164例非冠心病患者,采用PCR-酶切法检测PON1基因192位Gln-Arg多态性并进行对比分析。结果陕西地区汉族人群等位基因R和Q频率分别为60.23%和39.77%,其中CHD组RR、QR、QQ基因型分别占41.44%、39.64%、18.92%,对照组RR、QR、QQ基因型分别占35.98%、45.73%、18.29%;CHD组等位基因R频率占61.26%,Q频率占38.74%,对照组的等位基因R频率占58.84%,Q频率占41.56%,组间比较无显著性差异(P>0.05);心肌梗死和非心肌梗死、冠状动脉病变不同程度间等位基因R和Q频率及RR、QR、QQ基因型之间无显著性差异(P>0.05)。结论陕西汉族人群PON1192位Gln-Arg基因多态性与CHD发病之间无相关性。

Clinical study on the relationship between PON1 192 Gln-Arg polymorphism and Han patients with Coronary heart disease in Shaanxi

Objective To investigat the relationship between PON_1 192 Gln-Arg gene polymorphisms and coronary artery lesions in Shaanxi Han Chinese.Methods A total of 222 cases with one or more than two branches narrow(≥50%) and 164 cases with no coronary artery narrow(<30%),were diagnosed by coronary angiography.PON_1 192 Gln-Arg gene polymorphisms were tested by PCR-restriction enzyme digestion in these two groups and analyzed.Results The frequencies of Arg(R) and Gln(Q) in Han population of Shaanxi province were 60.23% and 39.77%,respectively.The frequencies of RR, QR and QQ genotypes of PON in CHD group were 41.44%,39.64% and 18.92%,respectively;and those in control group were 35.98%,45.73% and 18.29%,respectively;the frequencies of R and Q allele in CHD group were 61.26% and 38.74%,and those in control group were 58.84% and 41.56%.No differences were found in PON gene Gln-Arg polymorphisms between myocardial infarction and non infarction groups,and no differences were found among different narrow degrees of coronary artery(P>0.05).Conclusion There was no relationship between PON_(1) 192 Gln-Arg gene polymorphisms and Shaanxi Han patients with coronary heart disease.