陕南燃煤型砷中毒患者内外环境砷、硒水平及对血清P53、BCL-2、NO、MDA的影响

目的探讨陕南燃煤型砷中毒患者内外环境砷、硒水平及对血清P53、B淋巴细胞瘤/白血病-2(BCL-2)、一氧化氮(NO)、丙二醛(MDA)的影响。方法采用石墨炉原子吸收法测定煤、水、土、玉米、辣椒、血清、尿、发中砷、硒含量;采用ELISA法、硝酸还原酶法、TBA法分别测定血清P53、BCL-2、NO、MDA水平。结果病区煤、土、玉米、辣椒中砷、硒含量显著高于非病区;病例组血清、尿和发砷、硒含量显著高于内、外对照组;病例组血清BCL-2水平显著高于外对照组,病例组、内对照组的血清MDA水平显著低于外对照组。结论陕南燃煤型砷中毒患者内、外环境砷含量增高,外环境硒含量偏高而内环境硒负荷适宜,其血清BCL-2水平增高,MDA降低,而P53、NO无显著变化。

Arsenic and selenium levels in the internal and external environments and their effects on P53, BCL-2, NO and MDA in serum of burning-coal-type arseniasis patients from south Shaanxi Province

Objective To investigate the levels of arsenic(As) and selenium(Se) in the internal and external environment,and their effects on P53,B-cell lymphoma/leukemia-2(BCL-2),nitric oxide(NO) and maleic dialdehyde(MDA) in serum of burning-coal-type arseniasis from south Shaanxi Province.Methods As and Se contaminations in the external environment(coal,drinking water,soil,corns and pepper) and the internal one(serum,urine and hair) were analyzed using a graphite furnace atomic absorption spectrophotometer.The contents of P53,BCL-2,NO and MDA in serum were detected by ELISA,nitrifying ferment and TBA,respectively.Results The contents As and Se in coal,soil,corns and pepper from the arseniasis-affected area were significantly higher than those in the non-arseniasis-affected area.Concentrations of As in serum,urine and hair and Se in hair from patient group with arsenism were statistically higher than those from the other two groups.BCL-2 content in serum from patient group with arsenism was statistically higher than that from the controls in non-diseased areas,while MDA contents in serum from both patients and the control groups in the diseased areas were significantly lower than those from the controls in non-arsenism areas.Conclusion As levels in internal and external environment of patients with arsenism were statistically higher than those in the controls both arsenism-affected and non-arsenism-affected area,and Se levels in the external environment were increased while those in the internal were moderate.BCL-2 content was raised in the patients with arsenism,but MDA decreased,and P53 and NO had little changes.