| 牛关节软骨培养稳态模型的实验研究方法 |
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| 引用本文： | 郭传友,曹峻岭,张矢远.牛关节软骨培养稳态模型的实验研究方法[J].西安交通大学学报(医学版),1994(2). |
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| 作者姓名： | 郭传友 曹峻岭 张矢远 |
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| 作者单位： | 西安骨病研究所生化室，山东省滨州医学院附属医院外科 |
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| 摘 要： | 参照Ｈａｓｃａｌｌ实验方法建立了牛关节软骨体外培养，并通过组织学观察，３５Ｓ掺入率测定及已糖醛酸测定的方法证明在加入２０％小牛血清的ＤＭＥＭ培养液中，培养７ｄ后，软骨蛋白多糖的代谢达到了稳态，这为研究关节软骨蛋白多糖代谢及其影响因素提供了一个较好的实验模型。
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| 关 键 词： | 关节软骨，培养，蛋白多糖，稳态代谢 |
EXPERIMENTAL STUDY ON THE METHOD OF STEADY STATE METABOLISMOF BOVINE ARTICULAR CARTILAGE EXPLANT CULTURE |
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| Guo Chuanyou,Cao Junling,Zhang Shiyuan.EXPERIMENTAL STUDY ON THE METHOD OF STEADY STATE METABOLISMOF BOVINE ARTICULAR CARTILAGE EXPLANT CULTURE[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1994(2). |
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| Authors: | Guo Chuanyou Cao Junling Zhang Shiyuan |
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| Institution: | Biochemistry Lab of Institute of Bone Diseases |
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| Abstract: | This paper reports the establishment of bovine articular cartilage explant culture according to the method of Hascall.The determination of(35S)sulfate incorporation and hexuronate content was employed to monitor the proteoglycan metabolism state in culture. The growth condition of cartilage pieces was observed by upside and light microscope. It was found that a steady state metabolism of proteoglycan was reached at the 7th.day of culture.In this article,a profitable experimental model for the study of proteoglycan metabolism and regulation of articular cartilage is provided. |
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| Keywords: | articular cartilage culture proteoglycan steady state metabolism |
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