| Zfhag的锌指结构和同结构域的基因重组、表达和功能研究 |
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| 引用本文： | 朱本章.Zfhag的锌指结构和同结构域的基因重组、表达和功能研究[J].西安交通大学学报(医学版),1996(3). |
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| 作者姓名： | 朱本章 |
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| 作者单位： | 西安第一临床医学院内分泌科 |
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| 基金项目： | 美国国立卫生研究院资助项目 |
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| 摘 要： | 报道了成功地亚克隆Ｚｆｈａｇ的锌指结构和同结构域基因，并将其在细菌体内表达成麦芽糖结合蛋白的融合蛋白的方法和结果，使用这些表达蛋白进行了蛋白－ＤＮＡ相互作用的研究。结果表明：ＭＢＰ－ＺＤ１和ＭＢＰ－ＺＤ２蛋白能与糖蛋白激素的α亚基基因、鼠生长激素（γ－ＧＨ）基因和促甲状腺激素β亚基（ＴＳＨ－β）基因的甲状腺激素反应成分（ＴＲＥ）结合，而不能结合含有天然ＴＲＥ的ＭＨＣ和Ｆ２Ｈ基因，及含有合成ＴＲＥ的ＤＲ４和ＰＡＬ寡核苷酸。
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| 关 键 词： | Zfhag 融合蛋白 蛋白－DNA相互作用 转录因子 甲状腺激素 |
STUDIES ON THE RECOMBINATION AND EXPRESSION OF THE ZINC-FINGER HOMEODOMAIN GENES OF ZFHAG AND THE FUNCTION OF THE EXPRESSED PRODUCTS |
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| Zhu Benzhang,Douglas S. Darling.STUDIES ON THE RECOMBINATION AND EXPRESSION OF THE ZINC-FINGER HOMEODOMAIN GENES OF ZFHAG AND THE FUNCTION OF THE EXPRESSED PRODUCTS[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1996(3). |
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| Authors: | Zhu Benzhang Douglas S Darling |
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| Institution: | Department of Endocrinology First Affiliated Hospital |
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| Abstract: | The methods and results successfully subcloning the Zic-finger DNA binding domain and Homedomain genes of Zfhag,and successfully expressing them in bacteria as maltose-binding protein fusion proteins are reported in this article.These expressed proteins were used for research on protein-DNA interaction,The results showed that both MBP-ZD1 and ZD2 protein were able to bind to the glycoprotein hormone alpha-subunit gene, rGH and TSH-beta gene TRE, but not able to bind to the native TRE-containing MHC and F2H genes and synthetic TRE-containing DR4 and PAL oligonucleotides, The possible rote of Zfhag involved in the effecs of thyroid hormone on gene expression is discussed. |
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