牙龈炎、牙周炎患者龈沟液和血清中白细胞介素-8的含量及其与牙周指数的相关性

目的研究白细胞介素-8(IL-8)在牙周炎和牙龈炎中的来源,以及与临床指数间的相关性。方法采用双抗夹心酶联免疫吸附法(ELISA)测定成人牙周炎、牙龈炎患者及正常对照者龈沟液(GCF)及血清中的IL-8含量。结果①牙周炎患者GCF中IL-8含量明显高于对照组GCF中的含量(P<0.001)及牙周炎患者血清中的含量(P<0.001),与牙龈炎患者GCF中的含量无显著性差异(P>0.05);②牙龈炎患者GCF中IL-8含量高于对照组GCF中的含量(P<0.05);③牙周炎患者GCF中IL-8含量与探诊出血指数(BI)、牙周袋深度(PD)、附着丧失(AL)呈正相关关系(P<0.05)。结论IL-8主要来源于牙龈组织局部;在牙周炎的发生过程中IL-8并没有参与骨吸收;牙周炎患者GCF中IL-8水平与PD、BI、AL呈正相关关系。

Measurements of IL-8 in gingival crevicular fluid and in serum from patients with gingivitis and periodontitis and study of its correlation with clinical indexes

Objective To study the source of interleukin-8(IL-8) in gingivitis and periodontitis and its correlation with clinical indexes.Methods IL-8 contents in gingival crevicular fluid(GCF) were measured in periodontitis,gingivitis and healthy controls with sandwich enzyme-linked irnmunosorbent assay(ELISA).In addition,IL-8 contents in serum were studied in periodontitis and healthy controls at the same time.Results ① IL-8 level in GCF was markedly higher in periodontitis than in healthy controls(P<0.001) and than in serum of periodontitis(P<0.001).IL-8 level in GCF was not significantly different between adult periodontitis and gingivitis(P>0.05).② There was a significant difference of IL-8 in GCF between adult gingivitis and healthy controls(P<0.05).③ IL-8 level in GCF of the patients with periodontitis had a positive correlation with BI,PD and AL(P<0.05).Conclusion IL-8 in GCF is derived from local gingival tissues.IL-8 is not involved in bone absorption in the process of adult periodontitis.IL-8 level in GCF of patients with periodontitis has a positive correlation with BI,PD and AL.