新疆哈萨克族群体三个STR位点遗传多态性

目的　了解新疆哈萨克族人群D1 6S5 39,D7S82 0 ,D1 3S31 7三个STR位点的遗传多态性 ,建立该民族群体遗传学数据库。方法　运用复合PCR扩增 ,6 %变性聚丙烯酰胺凝胶电泳结合银染技术对 1 0 2位无关个体及 8个家系 42人的哈萨克族人群进行调查 ,并与其他种族或人群进行比较。结果　三个位点分别检测出 8、7、8个等位片段 ,多态性分布符合Hardy Weinberg平衡定律。期望杂合度为 0 .9439、0 .935 6、0 .930 4。三个位点的累积PIC =0 .990 5、DP =0 .9998、PE =0 .95 72。此外 ,在与其他四个人群比较中发现除与北京汉族在D7S82 0位点上无统计学意义 (P >0 .0 5 ) ,其余均可见显著性差异 (P <0 .0 5 )。同时 ,在家系调查中无一突变发现且均按孟德尔遗传规律传递。结论　三个STR位点的联合分析在法医学应用及群体遗传学中具有较高的价值。

Polymorphism of three STR loci in the Kazak ethnic population

ObjectiveTo analyze genetic polymorphism of D 16S539,D7S820 and D13S317 in Chinese Kazak ethnic population from Xinjiang and t o establish the population genetic database.MethodsOne hun dred and two unrelated individual and 8 samples of family (~{* ~}n~{* ~}=42) were invest igated by multiplex amlification and 6% denaturing PAGE and silver staining. A nd then the resultant allele frequencies were compared with other populations or race database.Results8,7,8 alleles were observed at these ST R loci respectively and the genotypes distributions were in accordance with Hard yWeinberg equilibrium. The expected heterozygosities for these loci were ~{*)~}0.9 439~{**~},0.9356 and 0.9304,the calculated PIC=0.9905,DP=0.9998,PE=0.9572. In additi on, the significant difference was found in comparison with other populations. Simul taneously, in the sample of families (~{* ~}n~{* ~}=42) no new mutation was found.ConclusionThis multiplexing of three STR loci can be used in f orensic identification and population genetics research.