弓形体普通冰箱保存方法试验

本文报道了4℃冰箱保存含弓形体的小鼠生理盐水腹腔洗涤液的保种方法,最长保存时间49天,仍具有感染力和再感染力,有效保存时间为35天。因此,一个月左右,通过一次动物转种,抽取小鼠生理盐水腹腔洗涤液,置4℃冰箱,如此重复,可以达到长期保种目的。此法与其它保种方法相比较,具有简便、易行等优点,值得试用。

THE METHOD TO PRESERVE TOXOPLASMA GONDII IN THE REFRIGERATOR

This paper reports a method to preserve toxoplasma gondii in the washing fluid of normal caline from the peritoneal cavity of mice in a refrigerator at 4℃. The washing fluid continued to infect the mice when it had been preserved for 49 days. Therefore, the mice san be vacunated through the peritoneal cavity about a month later, and then the washing fluid drawn from the peritoneal cavity and preserved in the refrigerator at 4℃ again. This method helps preserve toxoplasma gondii for a long time. This method is convenient, as compared with other methods.