白细胞介素-10启动区基因多态性与慢性丙型肝炎的相关性

目的　探讨白细胞介素 -10(IL- 10)启动区- 592 位点的基因多态性与慢性丙型肝炎的发生、发展以及转归的关系。方法　采用聚合酶链反应- 限制性片段长度多态性分析(PCR- RFLP)方法检测 85 例慢性丙型肝炎患者和 75 名健康对照者 IL- 10基因启动区域 592位点的基因型。结果　IL -10 基因启动区 592 位点基因型在慢性丙型肝炎患者与健康对照之间的分布无显著性差异(P> 0. 05); IL- 10 基因启动区 592 位点基因型在血清 HCV RNA> 105拷贝/mL组和HCV RNA≤105拷贝/mL组之间的分布无显著性差异(P>0.05);IL- 10 基因启动区 592 位点基因型在慢性丙型肝炎组和肝硬化组之间的分布无显著性差异(P>0.05); IL- 10 基因启动区 592 位点基因型在 ALT≥80U/L组中A/A基因型出现的比率高于ALT<80U/L组,两组之间分布存在显著性差异(P<0.01)。结论　IL- 10基因启动区域 592位点的基因多态性与慢性丙型肝炎和肝硬化患者的病毒血症水平无显著相关性;IL- 10 启动区域 592位点的基因多态性可能与慢性丙型肝炎患者的肝脏炎症反应有关。

Genetic polymorphisms of interleukin-10 promoter region in patients with chronic HCV infection

Objective To investigate the correlation between gene polymorphisms in IL-10 promoter region and the susceptibility and clinical phenotypes of HCV infection. Methods Two groups of subjects were studied. Group 1 included 85 treatment naive patients with chronic HCV infection, and group 2 included 75 healthy blood donors. Genotyping of IL-10 promoter-592 was performed by PCR-RFLP. Results No significant difference was found in IL-10 promoter-592 genotypes between the healthy individuals and HCV-infected patients. However, IL-10 promoter-592 genetype A/A appeared more frequently in patients with ALT≥80U/L than in those with ALT<80U/L group (P<0.01). Conclusion Genotype A/A appears more frequently in ALT≥80U/L patient group than in ALT<80U/L group, suggesting that genetic polymorphisms of interleukin-10 may be related to inflammatory reaction in the liver of the patients with chronic HCV infection.