| 一种新的胰腺炎脑损伤模型的建立 |
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| 引用本文： | 彭波,沙焕臣,马清涌.一种新的胰腺炎脑损伤模型的建立[J].西安交通大学学报(医学版),2010,31(2). |
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| 作者姓名： | 彭波 沙焕臣 马清涌 |
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| 作者单位： | 1. 西安交通大学医学院第一附属医院急诊科,陕西西安,710061
2. 西安交通大学医学院第一附属医院肝胆外科,陕西西安,710061 |
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| 基金项目： | 国家自然科学基金资助项目，陕西省科技攻关资助项目(No.2007k16-07)Supported by the National Natural Science Foundation of China，Shaanxi Science and Technology Task Force Project |
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| 摘 要： | 目的 建立一种稳定的胰腺炎并脑损伤模型,以进一步探讨胰腺炎脑损伤的机制.方法 24只成年雄性SD大鼠,随机分为假手术(sham operation, SO)组,重症急性胰腺炎(SAP)组和胰蛋白酶制模组(trypsin),每组8只大鼠.各组大鼠均于制模后12h采集脑组织和胰腺组织标本.比较各组大鼠的死亡率;采用酶联免疫吸附试验(enzyme linked immunosorbent assay, ELISA)测定血清细胞间紧密连接蛋白(Zo-1)含量;透射电镜观察脑组织超微结构变化;HE染色观察脑组织病理学变化.结果 SAP组大鼠死亡率较trypsin组明显升高,SO组无大鼠死亡;SAP组Zo-1水平均明显高于SO组(P<0.05),trypsin组Zo-1水平与SAP组呈现一致性变化(P>0.05).SAP组和trypsin组均出现明显神经元细胞肿胀、毛细血管淤血、血管通透性增加、血栓形成、线粒体肿胀和细胞凋亡等超微结构的变化.结论 胰蛋白酶可能为SAP脑损伤的元凶;用胰蛋白酶制备SAP模型大鼠死亡率低,为进一步研究脑损伤的机制和进行相关治疗研究提供了稳定的动物模型.
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| 关 键 词： | 重症急性胰腺炎 脑损伤模型 胰蛋白酶 |
Establishment of a new brain injury model with pancreatitis |
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| PENG Bo,SHA Huan-chen,MA Qing-yong.Establishment of a new brain injury model with pancreatitis[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2010,31(2). |
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| Authors: | PENG Bo SHA Huan-chen MA Qing-yong |
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| Institution: | PENG Bo1,SHA Huan-chen2,MA Qing-yong2(1.Department of Emergency,2.Department of Hepatobiliary Surgery,the First Affiliated Hospital,Medical School of Xi\'an Jiaotong University,Xi\'an 710061,China) |
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| Abstract: | Objective To establish a stable brain injury model with pancreatitis and explore the mechanism of brain injury resulting from severe acute pancreatitis(SAP) in experimental rat models.Methods Twenty-four adult male Sprague-Dawley rats were randomly divided into three groups: sham operation(SO) group,SAP group and trypsin group,with eight rats in each.Brain tissue and pancreas tissue specimens were collected at 12 h after treatment.Death rate in each group was evaluated;the level of tight junction protein zo... |
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| Keywords: | Zo-1 severe acute pancreatitis brain injury model trypsin Zo-1 |
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