| 宫颈癌组织表皮生长因子受体对TKI敏感性相关的基因突变研究 |
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| 引用本文： | 薛琴琴,张菊,俞青苗,金雯,杨梅,刘宁侠,高艳娥. 宫颈癌组织表皮生长因子受体对TKI敏感性相关的基因突变研究[J]. 西安交通大学学报(医学版), 2012, 33(5): 583-586,592 |
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| 作者姓名： | 薛琴琴 张菊 俞青苗 金雯 杨梅 刘宁侠 高艳娥 |
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| 作者单位： | 西安交通大学医学院第二附属医院妇产科;榆林市第一医院妇产科;第四军医大学药学系基因所 |
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| 摘 要： | 目的研究宫颈癌组织表皮生长因子受体(epidermal growth factor receptor,EGFR)基因中是否存在与EGFR酪氨酸激酶抑制剂(tyrosine kinase inhibitor,TKI)药物敏感性相关的18、19及21外显子突变,为本地区宫颈癌的靶向治疗提供依据。方法收集70例宫颈癌患者的新鲜癌组织标本,提取DNA,以特异性引物PCR扩增EGFR基因外显子18、19及21,进行DNA测序并分析序列相似性。结果 70例宫颈癌组织提取质量良好的DNA中,均未检测到EGFR基因18、19及21外显子突变。结论宫颈癌组织EGFR基因外显子18、19及21突变罕见。
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| 关 键 词： | 宫颈癌 表皮生长因子受体 基因突变 多聚酶链式反应 DNA测序 |
EGFR TKI-sensitive gene mutation in cervical cancer |
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| XUE Qin-qin,ZHANG Ju,YU Qing-miao,JIN Wen,YANG Mei,LIU Ning-xia,GAO Yan-e. EGFR TKI-sensitive gene mutation in cervical cancer[J]. Journal of Xi‘an Jiaotong University:Medical Sciences, 2012, 33(5): 583-586,592 |
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| Authors: | XUE Qin-qin ZHANG Ju YU Qing-miao JIN Wen YANG Mei LIU Ning-xia GAO Yan-e |
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| Affiliation: | 1(1.Department of Obstetrics and Gynecology,the Second Affiliated Hospital, Medical School of Xi’an Jiaotong University,Xi’an 710004;2.Department of Obstetrics and Gynecology,Yulin First Hospital,Yulin 719000;3.Institute of Gene Diagnosis, Department of Pharmacology of Fourth Military Medical University,Xi’an 710032,China) |
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| Abstract: | Objective To detect whether in epidermal growth factor receptor(EGFR) there exists mutation of exons 18,19 and 21 associated with tyrosine kinase inhibitor(TKI) medication-sensitivity in cervical cancer patients for the purpose of providing evidence for targeting treatment of cervical cancer in the region.Methods Fresh tissue specimens were collected from 70 patients with cervical cancer and DNA was extracted from these specimens.Exons 18,19 and 21 of EGFR were amplified with designed specific primers and DNA templates extracted from cervical cancer tissues.Then,the PCR products were sequenced on DNA sequencer,and the sequences were BLAST analyzed.Results No mutations were detected in exons 18,19 or 21 of EGFR in the 70 human cervical cancer samples analyzed.Conclusion Mutations in exons 18,19 and 21 of EGFR are very rare in cervical cancer. |
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| Keywords: | cervical cancer epidermal growth factor receptor gene mutation polymerase chain reaction(PCR) DNA sequencing |
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