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三阴性乳腺癌差异表达miRNA的筛选
引用本文:赵晓艾,胡金华,赵新汉.三阴性乳腺癌差异表达miRNA的筛选[J].西安交通大学学报(医学版),2012,33(5):568-571.
作者姓名:赵晓艾  胡金华  赵新汉
作者单位:西安交通大学医学院第一附属医院肿瘤内科,陕西西安,710061
基金项目:陕西省科学技术研究发展计划项目(社发攻关)(No.2010K14-01)~~
摘    要:目的应用miRNA芯片技术筛选三阴性乳腺癌及癌旁组织差异表达的miRNAs,为进一步阐明其在三阴性乳腺癌发病机制中的作用奠定基础。方法收集8例新鲜的三阴性乳腺癌及其癌旁组织,抽提总RNA进行Hy3荧光标记,miRCURYTMLNA Array(v.16.0)(Exiqon)芯片上进行杂交反应,实时定量PCR验证miRNAs芯片结果的可靠性。结果对芯片进行图像扫描和数据分析,筛选获得17个三阴性乳腺癌相关miRNAs,其中7个miRNAs表达上调,10个miRNAs表达下调;显著上调的有miR-93、miR-328,显著下调的有miR-145、miR-205。结论筛选得到三阴性乳腺癌miRNA差异表达谱,其可能与三阴性乳腺癌的发生发展有关。

关 键 词:miRNA  三阴性乳腺癌  miRNA芯片  实时定量PCR

MiRNA expression profile in triple-negative breast cancer
ZHAO Xiao-ai , HU Jin-hua , ZHAO Xin-han.MiRNA expression profile in triple-negative breast cancer[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2012,33(5):568-571.
Authors:ZHAO Xiao-ai  HU Jin-hua  ZHAO Xin-han
Institution:(Department of Medical Oncology,the First Affiliated Hospital, Medical School of Xi’an Jiaotong University,Xi’an 710061,China)
Abstract:Objective To screen and identify the miRNA differential expression profile in triple-negative breast cancer by the miRNA array technique.Methods Total RNA was extracted from 8 fresh breast tumor samples and normal adjacent tissues.The miRCURYTM LNA Array(v.16.0)(Exiqon) was used to screen the differentially expressed miRNA.Real-time quantitative PCR was applied to verify the reliability of miRNA array results.Results Compared to normal adjacent tissues,17 miRNAs were differentially expressed in triple-negative breast cancer tissues,including 7 up-regulated and 10 down-regulated miRNAs.The most significantly up-regulated miRNAs were miR-93 and miR-328,while miR-145 and miR-205 were significantly down-regulated.Conclusion We obtained miRNA differential expression profile of triple-negative breast cancer.The profile may be related to the pathogenesis and development of triple-negative breast cancer.
Keywords:miRNA  triple-negative breast cancer  miRNA array  real-time quantitative PCR
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