纳米氧化锌对大鼠肝及肝癌细胞的毒性效应

目的研究氧化锌纳米颗粒引致大鼠肝细胞(BRL-3A)和大鼠肝癌细胞(CBRH-7919)毒性的潜在机制。方法不同浓度(0.1～100mg/L)的氧化锌纳米颗粒与细胞相互作用不同时间(12～48h)后,检测细胞的生存率及细胞生成的活性氧(ROS)和谷胱甘肽(GSH)浓度的变化。结果氧化锌纳米颗粒以浓度依赖模式及时间依赖模式诱导细胞毒性,且大鼠肝癌细胞对氧化锌纳米颗粒的耐受性好于正常大鼠肝细胞;ROS的浓度变化与GSH浓度变化的关系及其与细胞生存率变化的关系都呈负相关,即氧化锌纳米颗粒诱导的细胞毒性是通过氧化应激产生作用的。结论本研究揭示了纳米颗粒诱导的细胞毒性在不同种类的细胞间存在差异,这一结果有利于准确评估纳米材料对生物机体的整体毒性效应,并为应用纳米材料科学、合理地治疗肿瘤提供了依据。

Cytotoxicity of ZnO nanoparticles in rat liver cells and hepatocarcinoma cells

Objective To explore the potential cytotoxicity mechanisms of ZnO nanoparticles(NPs) in BRL-3A(rat liver cell line) and CBRH-7919(rat hepatocarcinoma cell line) cells.Methods We checked cell viability after ZnO exposure at varying concentrations(0.1-100 mg/L) and different exposure periods(12-48 h) and changes in reactive oxygen species(ROS) and glutathione(GSH) levels.Results Compared to the NP-free controls,ZnO NPs induced cytotoxicity in concentration-dependent and time-dependent manners in both cell lines.We found high cell viability in CBRH-7919 cells,indicating the better tolerance of CBRH-7919 cells to ZnO NPs than to BRL-3A cells.The increased ROS levels had a negative correlation with reduced cell viability and GSH levels,indicating that ZnO NPs could lead to cytotoxicity through oxidative stress in normal and cancer cells.Conclusion These results suggest that there exist different degrees of cytotoxicity induced by ZnO NPs in normal and cancer cells,which may deserve consideration in future assessment of nanotoxicity and treatment of cancer.