3-吗啉丙磺酸缓冲的生理盐溶液的配制及pH稳定性

目的配制以3-吗啉丙磺酸和Na2HPO4为缓冲对的生理盐溶液(MOPS液),并观察其pH稳定性及对大鼠离体血管张力的影响。方法配制MOPS液和Krebs液及其母液,分别给MOPS液或Krebs液通O2或950mL/L O2+50mL/L CO2,用pH计测定pH值,用张力描记仪记录大鼠离体血管的张力。结果 MOPS液和K+-MOPS液14d内的pH变化为(7.32±0.04)～(7.51±0.04),基本恒定;Krebs液和K+-Krebs液随时间延长,pH由7.40±0.01逐渐升至8.41±0.04。通气速度对MOPS液的pH影响小,变化范围为(7.24±0.04)～(7.39±0.00);Krebs液对通气速度敏感,在15～60个气泡/min,pH的变化范围在(6.81±0.07)～(8.33±0.02)之间,波动范围较大。在相同通气速度下MOPS液和Krebs液体积的变化对其pH影响小。大鼠离体的肠系膜动脉、脑基底动脉和冠状动脉在MOPS液中能产生良好的收缩。结论 MOPS液具有pH稳定、放置时间长、通气容易掌握,并且应用成本低的优点,可用于哺乳动物离体组织、器官如血管等实验。

Preparation and pH stability of a physiological salt solution buffered with 3-Morpholine propanesulfonic acid

Objective To prepare a normal saline solution buffered with 3-morpholine propanesulfonic acid(MOPS),and to study its pH stability and its influence on rat artery rings isometric tension.Methods The MOPS solution,Krebs solution and their stock solutions were prepared,and aerated with O2 or 95% O2+5% CO2.The solutions’ pH was measured by pH meter,and an isometric tension of rat artery rings was recorded by a sensitive myograph in vitro.Results The pH value of MOPS solution and K+-MOPS solution ranged steadily from 7.32±0.04 to 7.51±0.04 in 14 days.The pH value of Krebs solution and K+-Krebs solution increased gradually from 7.40±0.01 to 8.41±0.04 over time and extended,and its fluctuation range was large.Aerating velocity had little effect on the pH of MOPS solution,which ranged from 7.24±0.04 to 7.39±0.00;while the pH value of Krebs solution was sensitive to aerating velocity,ranging from 6.81±0.07 to 8.33±0.02 with a velocity of 15-60 bubbles per minute.The volume of solutions had little effect on the pH values with the same velocity of aeration.The contractility of rat mesenteric artery,cerebral basal artery and coronary artery in MOPS solution was satisfactory.Conclusion The MOPS normal saline solution has the advantages of longer-time storage,more stable pH,easier aerating and lower cost.It can be used for experiments on mammalian organs and tissues such as artery in vitro.