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Insulin-3基因敲除鼠生殖系统LGR8蛋白的表达
引用本文:王毅,张少华,刘娟,BAKER Linda.Insulin-3基因敲除鼠生殖系统LGR8蛋白的表达[J].西安交通大学学报(医学版),2012,33(2):172-175.
作者姓名:王毅  张少华  刘娟  BAKER Linda
作者单位:1. 西安交通大学医学院第一附属医院内分泌科,陕西西安,710061
2. Department of Urology, UT Southwestern Medical Center at Dallas, Dallas, Texas, USA 75235
摘    要:目的 Insulin-3(Insl3)和其唯一配体富含亮氨酸的G蛋白受体LGR8(aka RXFP2)]系统在睾丸下降过程中起重要作用。本研究拟利用Insl3基因敲除鼠探讨Insl3与LGR8相互关系及其在生殖系统的表达情况。方法使用免疫共沉淀、Western blot及免疫组化法检测雄性野生型小鼠和Insl3基因敲除雄性小鼠各生殖器官LGR8蛋白的表达并比较分析。结果 LGR8蛋白在野生型小鼠睾丸、睾丸引带、附睾及输精管等多个雄性生殖器官均有表达。其表达程度在不同生殖器官不同,野生型小鼠睾丸LGR8的表达强于Insl3基因敲除鼠(P<0.05)。结论 LGR8蛋白在多个雄性生殖器官均有表达,Insl3-LGR8系统可能在男性多种生殖发育疾病中起作用。

关 键 词:胰岛素样因子3  富含亮氨酸的G蛋白受体  睾丸下降  隐睾症  男性生殖发育疾病

Expression of LGR8 protein in the reproduction system in insulin-3 knockout mice
WANG Yi , ZHANG Shao-hua , LIU Juan , BAKER Linda.Expression of LGR8 protein in the reproduction system in insulin-3 knockout mice[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2012,33(2):172-175.
Authors:WANG Yi  ZHANG Shao-hua  LIU Juan  BAKER Linda
Institution:WANG Yi1,ZHANG Shao-hua2,LIU Juan1,BAKER Linda2(1.Department of Endocrinology,the First Affiliated Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710061;2.Department of Urology of UT Southwestern Medical Center at Dallas,Dallas 75235,USA)
Abstract:Objective Insulin-3(Insl3) and its only receptor LGR8(aka RXFP2) are the key mediators in testicular descent.We sought to characterize LGR8 localization in the reproduction system of Insl3 knockout mice to better understand infertility seen in these mice. Methods IP,Western blot and immunohistochemistry techniques were used to detect the expression of LGR8 protein in the reproduction system of Insl3 knockout mice. Results LGR8 was found to be expressed in the testis,gubernaculum,epididymis and vas deferens in wildtype mice.The expression of LGR8 varied in different organs,and it was significantly higher in the testis of wildtype mice than in that of Insl3 knockout mice(P<0.05). Conclusion LGR8 is expressed in the reproduction system of male mice,indicating that Insl3-LGR8 system may play an important role in male infertility.
Keywords:insulin-3  leucine-rich repeat-containing G protein-coupled receptor 8(LGR8)  testicular descent  cryptorchidism  male infertility diseases
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