| Cloning, Sequencing, and Characterization of Porcine Sterol Regulatory Element Binding Proteins-1c Gene |
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| 作者姓名： | 李长龙 潘玉春 孟和 李婧 王起山 |
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| 作者单位： | School of Agriculture and Biology Shanghai Jiaotong Univ. Shanghai 201101 China，School of Pharmacy East China Univ. of Science and Technology Shanghai 200237，School of Agriculture and Biology Shanghai Jiaotong Univ. Shanghai 201101 China，School of Agriculture and Biology Shanghai Jiaotong Univ. Shanghai 201101 China，School of Agriculture and Biology Shanghai Jiaotong Univ. Shanghai 201101 China，School of Agriculture and Biology Shanghai Jiaotong Univ. Shanghai 201101 China |
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| 基金项目： | Item supported by national“973”program(No. 2004CB117500)，national natural science foundation(No.30471237) |
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| 摘 要： |
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Cloning, Sequencing, and Characterization of Porcine Sterol Regulatory Element Binding Proteins-1c Gene |
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| LI Chang-long.Cloning, Sequencing, and Characterization of Porcine Sterol Regulatory Element Binding Proteins-1c Gene[J].Journal of Shanghai Jiaotong university,2005(Z1). |
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| Authors: | LI Chang-long |
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| Institution: | LI Chang-long~ |
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| Abstract: | Primers were designed according to the known sequences of Sterol Regulatory Element Binding Proteins-1c (SREBP-1c) genes of human, rat and pig. RT-PCR was then used to amplify porcine SREBP-1c gene with total RNA of spleen tissue. A 760 bp segment of cDNA was cloned and sequenced. Homogeneous comparison showed that the sequence of porcine SREBP-1c had 99.9% and 99.1% homogeneity with the two known partial mRNA sequences of porcine SREBP-1c gene. The complete cDNA was obtained mainly based on the known partial sequences, which has 3 830 bp, encoding 1 151 amino acids with a calculated molecular weight of 121 479 Da. It is the first time that we get complete encode sequence of porcine SREBP-1c gene. The complete cDNA sequence has high homogeneity with SREBP-1c gene of other species. A characteristic structure of HLH (Helix-Loop-Helix) and four transmembrane segments were found in the amino acids. The sequence had been submitted to GenBank (Accession No.NM_-214157). |
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| Keywords: | pig SREBP-1c RT-PCR cloning sequence analysis NM_-214157 |
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