荧光原位杂交法与免疫组织化学法检测乳腺癌c-erbB-2表达的比较

目的 比较免疫组织化学法(IHC)和荧光原位杂交法(FISH)检测乳腺癌c-erbB-2状态的一致性.方法 选取乳腺癌石蜡标本共50例,其中以IHC法检测c-erbB-2蛋白表达(+)10例、(++)20例、(+++)20例.以FISH法检测上述乳腺癌细胞c-erbB-2基因扩增情况,分析比较两种方法差异性与相关性.结果 在IHC检测乳腺癌c-erbB-2(+)和(+++)时,FISH检测结果与其具有较好的一致性,总符合率为89.2%.在IHC检测c-erbB-2(++)时,FISH结果与其一致性较差,符合率仅为35.3%.结论 IHC可以作为初步筛查c-erbB-2表达的首选方法;IHC检测乳腺癌c-erbB-2(+)和(+++)时与FISH有良好的一致性.因IHC检测c-erbB-2表达(++)时与FISH结果的一致性较差,这类患者在应用赫赛汀治疗时应做FISH确诊.

Comparison of immunohistochemistry and fluorescence in situ hybridization in detecting c-erbB-2 expression in breast cancer

Objective To compare the consistency of immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) in detecting c-erbB-2 status in breast cancer tissues. Methods A total of 50 breast cancer paraffin embedded samples were selected, of which there were 10 cases of c-erbB-2 protein expression (+), 20 cases of (++) and 20 cases of (+++). FISH was used to assess the amplification of c-erbB-2 gene, and SPSS 13.0 software was employed to analyze the difference and consistency between the two methods. Results IHC and FISH methods had a good consistency when detecting c-erbB-2 (+) and (+++) expressions in breast cancer tissues, with the coincidence rate of 89.2%. However, when IHC was used to test c-erbB-2 (++), the result of FISH was quite different, with the coincidence rate of only 35.3%. Conclusion IHC is a preliminary method to detect c-erbB-2 status in breast cancer. IHC and FISH methods have a good consistency in detecting c-erbB-2 (+) and (+++) status in breast cancer tissues. As detection of c-erbB-2 (++) with IHC has a different result from FISH, such patients should receive FISH confirmation for herceptin therapy.