| 肝硬化组织微卫星改变的临床研究 |
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| 引用本文： | 姚英民,王小娟,吕春华,刘青光.肝硬化组织微卫星改变的临床研究[J].西安交通大学学报(医学版),2003,24(3):205-206,210. |
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| 作者姓名： | 姚英民 王小娟 吕春华 刘青光 |
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| 作者单位： | 西安交通大学第一医院肝胆外科,陕西西安,710061 |
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| 摘 要： | 目的 研究微卫星改变 (microsatellitealteration ,MA)在肝硬化组织的发生情况及其与病毒性肝炎的关系。方法 运用聚合酶链式反应 -简单重复序列多态性方法 (PCR SSLP)对 30例肝硬化组织石蜡标本的微卫星不稳定性 (microsatelliteinstability ,MSI)和等位基因杂合性丢失 (lossofheterozygosity ,LOH)进行研究。 结果 30例肝硬化组织中有 17例出现了MA(5 6 .7% ) ,其中至少有一个位点发生的MSI有 4例 (13.3% ) ,而有 15例至少有一个位点出现了LOH(5 0 % ) ,MA的发生与HBV感染及是否合并HCV感染无显著相关。结论 肝硬化作为肝癌的早期表现就已经存在有微卫星改变
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| 关 键 词： | 肝硬化 微卫星不稳定性 等位基因杂合性丢失 |
| 文章编号： | 1671-8259(2003)03-0205-02 |
The clinical study of microsatellite alteration of liver cirrhosis |
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| Abstract: | Objective To investigate the frequency of microsatellite alteration (MA) in cirrhotic livers and explore its association with viral hepatitis. Methods The loss of heterozygosity (LOH) and microsatellite instability (MSI) were analysed in 30 paraffin embedded cirrhotic tissues by polymerase chain reaction simple sequence length polymorphism (PCR SSLP). Results Out of 30 cirrhotic tissues, MA was detected in 17 (56.7%) , MSI was observed in 4 and LOH was observed in 15 at one or more loci. No significant correlation was found between MA and HBV and HCV infection. Conclusion MA occurs in cirrhosis as the early lesion of hepatocellular carcinoma. |
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| Keywords: | liver cirrhosis microsatellite instability loss of heterozygosity |
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