| EXPRESSION OF HUMAN RECOMBINANT INTERLEUKIN-1 RECEPTOR ANTAGONIST (rhIL-1ra) IN E.COLI |
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| 作者姓名： | 耿捷 楚雍烈 付伟 张霄瑜 |
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| 作者单位： | Department of Microbiology，Xi'an Medical University，Xi'an 710061 |
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| 摘 要： | ThisprojectissupportedbyChineseMedicalBoard(U.S.A)In1990,EisenbergisolatedacDNAfromthehumanmonocytelibrary,expressionofthecDNAinE.coltyieldedtheproteinthatcouldhinderIL--lbindingIL--lreceptor,hetermedtheproteininterleukin--1receptorantagonist(IL--lra)ti.23.IL--lraisauniquecytokinethatcanstronglybindthecellsurfacereceptorsofIL--Iwithoutinducinganydetectableintracellularresponses"'.SinceitisaneffectivecompetitiveinhibitorofIL--lbothinvitroandinvivot'3,IL--lraisausefultooltodetermineth…
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EXPRESSION OF HUMAN RECOMBINANT INTERLEUKIN-1 RECEPTOR ANTAGONIST (rhIL-1ra) IN E. COLI |
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| Geng Jie,Chu Yonglie,Fu Wei,Zhang Xiaoyu.EXPRESSION OF HUMAN RECOMBINANT INTERLEUKIN-1 RECEPTOR ANTAGONIST (rhIL-1ra) IN E.COLI[J].Academic Journal of Xi’an Jiaotong University,1998(1). |
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| Authors: | Geng Jie Chu Yonglie Fu Wei Zhang Xiaoyu |
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| Abstract: | Using restriction endonuclease Hind,. Sal i, the IL-lra cDNA was isolated from PRCra, then by a medium plasmid pUC19, It was cloned into a high level expressive vector PBV220. Therecombinant plasmid pBV-IL--Ira waS transrormed Into E. coil DH-So. After induced by temperaIure, a 20KD protein could be detected by SDS-PAGE. Biological assay in vitro showed that the protein could inhibit the activity or IL-l obviously. |
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| Keywords: | IL-1ra cloning expression genetic engineering |
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