| 髓样细胞白血病-1基因在多发性骨髓瘤发病中的分子机制 |
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| 引用本文： | 王孟昌,张斌. 髓样细胞白血病-1基因在多发性骨髓瘤发病中的分子机制[J]. 西安交通大学学报(医学版), 2011, 32(4): 429-432,448 |
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| 作者姓名： | 王孟昌 张斌 |
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| 作者单位： | 1. 西安交通大学医学院第一附属医院血液科,陕西西安,710061
2. 美国Maryland癌症中心,巴尔的摩21205 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的通过检测骨髓瘤细胞中依赖IL-6与否的髓样细胞白血病-1(mcl-1)基因的表达,阐明mcl-1基因在多发性骨髓瘤发病中的信号转导通路。方法应用JAK特异性抑制剂AG490、蛋白激酶抑制剂LY294002、ERK1/2信号通路的特异性阻断剂PD98059,分别研究了JAK/STAT通路、ras/MAPK通路及PI3K/Akt三大信号途径与mcl-1基因在多发性骨髓瘤中的关系。结果在骨髓瘤细胞8226和U266中,抑制ERK1/2活性不能改变mcl-1的表达;高浓度的AG490不能抑制STAT3的酪氨酸磷酸化;LY294002可抑制8226细胞和ANBL-6细胞的增殖,但不抑制mcl-1的表达。结论 Ras/MAPK通路以及PI3K通路在mcl-1基因的调节中无作用,但STAT的作用仍然需要进一步探讨。
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| 关 键 词： | 髓样细胞白血病-1(mcl-1) 多发性骨髓瘤 IL-6 信号转导 |
Molecular mechanism of myeloid cell leukemia-1 for multiple myeloma |
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| WANG Meng-chang,ZHANG Bin. Molecular mechanism of myeloid cell leukemia-1 for multiple myeloma[J]. Journal of Xi‘an Jiaotong University:Medical Sciences, 2011, 32(4): 429-432,448 |
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| Authors: | WANG Meng-chang ZHANG Bin |
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| Affiliation: | WANG Meng-chang1,ZHANG Bin2(1.Department of Hematology,the First Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710061,China,2.University of Maryland Cancer Center,Baltimore 21205,USA) |
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| Abstract: | Objective To clarify the signal transduction pathway of myeloid cell leukemia-1(mcl-1) gene in the pathogenesis of multiple myeloma via studying the mcl-1 gene expression of dependent and independent IL-6 in myeloma cells.Methods The JAK inhibitor AG490,protein kinase inhibitor LY294002,and ERK1/2 signaling pathway specific inhibitor PD98059 were used to explore the relationship between mcl-1 gene and the three signal pathways: JAK/STAT,Ras/MAPK and PI3K/Akt in multiple myeloma.Results In multiple myeloma c... |
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| Keywords: | IL-6 |
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