Target-to-background separation for spectral unmixing in in-vivo fluorescence imaging |
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Authors: | Yong Zhao Cheng Hu Jin-liang Peng Bin-jie Qin |
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Institution: | 1. School of Biomedical Engineering, Shanghai Jiaotong University, Shanghai, 200240, China
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Abstract: | We present a novel fluorescence spectral unmixing based on target-to-background separation preprocessing, which effectively separates the multi-target fluorescence from all background autofluorescence (BF) without any hardware-based BF acquisition and tissue specific BF estimation. Specifically, we first enhance the intrinsic accumulation contrast in target-to-background fluorescence using h-dome transformation; then separate multi-target fluorescence areas from the background in sparse multispectral data utilizing kernel maximum autocorrelation factor analysis; we further use fast marching-based image inpainting method to patch up the removed target fluorescence areas and reconstruct the multispectral BF; with the BF matrix being subtracted from the original data, the multi-target fluorophores are easily unmixed from the subtracted data using multivariate curve resolution-alternating least squares method. In two preliminary in-vivo experiments, the proposed method demonstrated excellent performance to unmix multi-target fluorescences while other state-of-art unmixing methods failed to get desired results. |
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