基因芯片技术在COX10等无精症患者相关基因的分析

目的　运用基因芯片技术获取正常生育者睾丸组织与无精症患者睾丸组织中差异表达的基因 ,并对结果进行生物信息学分析。方法　分别抽提正常生育者与无精症患者睾丸组织中mRNA制备探针 ,应用基因芯片技术观察二者表达谱差异情况 ,并对其中明显下调的COX10基因进行生物信息学分析。结果　通过人基因芯片筛选 ,获得 12 0条与无精症相关的基因 ,其中明显下调的基因COX10与人类精子调控关系密切。结论　基因芯片筛选正常生育者与无精症患者睾丸组织差异表达基因具有样品用量少 ,高敏感性 ,快速高效等特性 ,可用于筛选和研究无精症相关基因

The study on the aspermia-related genes by genechips and bioinformatics analysis of COX10 gene

Objective To study the differential gene expression profiles between the normal and aspermia human testises by genechips and analyze the biological information of COX10, a distinct down expressed gene. Methods We extracted mRNA from both the normal and aspermia testises and produced probes to detect the differential gene expression profiles by genechips, then analyzed COX10 genes by bibliographic retrieval on the Internet. Results One hundred and twenty differentially expressed genes related to aspermia were found. A distinct down-expressed gene COX10 was confirmed to be related to aspermia by bibliographic retrieval. Conclusion Screening the differential gene expression profiles between the normal and aspermia human testises by genechips is fast and effective, and needs less swatch than normal technique. It can be used in the study of aspermia-related genes and further research.