LEPTIN STIMULATING PROLIFERATION AND DIFFERENTIATION OF HUMAN OSTEOBLAST-LIKE CELL LINE MG63 IN DOSE-DEPENDENT AND TIME-DEPENDENT MANNERS |
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| Authors: | PENG Mian YU Xue-tao CHEN Shu CAI Xiao-hua PENG Yi-chao PENG Xiao-rong |
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| Institution: | PENG Mian(First Affiliated Hospital of Shantou Medical College, Shantou 515041, China)；YU Xue-tao(First Affiliated Hospital of Shantou Medical College, Shantou 515041, China)；CHEN Shu(Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, China)；CAI Xiao-hua(Puning People's Hospital, Puning 515300, China)；PENG Yi-chao(Puning People's Hospital, Puning 515300, China)；PENG Xiao-rong(Puning People's Hospital, Puning 515300, China)； |
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| Abstract: | Objective To investigate the direct effect of leptin on osteoblast-like cell line MG63. Methods Human osteoblast-like cell line MG63 was incubated with leptin of different doses for 24, 48 and 72 h, respectively.The proliferation of MG63 was determined by methylene blue assay. Alpha1 (Ⅰ) collagen gene expression in MG63was determined by real time flourescence quantitive PCR (FQ-PCR), both with 17β-E2 as positive control.Results Leptin accelerated the proliferation and differentiation of MG63 in dose and time-dependent manners,with the best effect at 10-7 mol/L at 72 h. Compared with 17β-E2 , leptin showed a weaker promoting effect at all of the three time point: 24, 48 and 72 h. While the effects of the two hormones have an approaching trend the time prolonged. Conclusion Leptin has the effects of accelerating the proliferation and differentiation of MG63 cells in vitro, which is more enduring and later than that of 17β-E2. |
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