| 用流式细胞测定仪测定AITD患者TRAb |
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| 引用本文： | 王琛,雒文田,徐利,程晓丽,马秀萍,高慧.用流式细胞测定仪测定AITD患者TRAb[J].西安交通大学学报(医学版),1999(1). |
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| 作者姓名： | 王琛 雒文田 徐利 程晓丽 马秀萍 高慧 |
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| 作者单位： | 第一临床医学院内分泌代谢病研究室!西安７１００６１(王琛，雒文田，徐利，马秀萍，高慧)，第一临床医学院中心实验室(程晓丽) |
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| 摘 要： | 以人工重组ＴＳＨ受体表达的中国仓鼠卵细胞（ＣＨＯ－ＴＳＨＲ）为靶细胞，用流式细胞（ＦＡＣＳ）测定技术，对４５例自身免疫性甲状腺病（ＡＩＴＤ）患者血清中ＳＨ受体抗体（ＴＲＡｂ）进行测定。结果显示：３６例ＧＤ患者阳性细胞结合的百分数为１．９％～７７．４％。阳性率为９１．６６％。其均值２６．１３９％与正常人均值６．１６３％相比有显著性差异（Ｐ＝０．０００１）。９例桥本（ＨＴ）甲减患者５例阳性，阳性率为５５．５６％。证实以ＣＨＯ－ＴＳＨＲ细胞为靶细胞用ＦＡＣＳ进行ＴＲＡｂ汕测定不失为一种敏感简便的新方法。
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| 关 键 词： | TRAb FACS CHO－TSHR Graves’病 |
FLOW CYTOMETRIC ANALYSES OF TRAb WITH AITD |
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| Wang Chen, Luo Wentian, Xiu Li et al.FLOW CYTOMETRIC ANALYSES OF TRAb WITH AITD[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1999(1). |
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| Authors: | Wang Chen Luo Wentian Xiu Li |
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| Abstract: | Using Chinese hamster ovary(CHO) cells that express high numbers of TSH receptor (TSHR) on the surface, we studied bindingactivity of antibodies in patients' sera to the TSHRby flow cytometry. It showed thatthe binding activity of 36 sera from active Graves' disease patientswas. 1.9%~77. 4%, while 30 sera from normalsubjects was 0. 1% ~15. 1 %. The positive rate was91. 66%. The median binding activity in activeGraves' disease patients (26. 139% ) was significantly higher than that of normal subjects (6. 163%;P=0. 0001). We also studied 9 sera from Hoshimototyroiditis (HT). The positive rate was 55. 56%. Inconclusion, this method can be used to detect binding of antibodies to TSHR. |
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| Keywords: | TSH receptor antibodies (TRAb) flow cytometric analyses (FACS) Chinese hamster ovarycells-TSH receptor (CHO-TSHR) Gravea' disease (GD) |
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