| A NORTHWEST DATABASE MODEL OF SHORT TANDEM REPEAT LOCI IN FORENSIC MEDICINE |
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| 引用本文： | 王振原,朱波峰,刘雅诚,严江伟,霍振义,金天博,李涛,樊拴良,方杰. A NORTHWEST DATABASE MODEL OF SHORT TANDEM REPEAT LOCI IN FORENSIC MEDICINE[J]. 西安交通大学学报(英文版), 2003, 15(1) |
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| 作者姓名： | 王振原 朱波峰 刘雅诚 严江伟 霍振义 金天博 李涛 樊拴良 方杰 |
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| 作者单位： | Department of Forensic Medicine，Department of Forensic Medicine，Forensic Medical Examination and Identifican Center of Beijing Public Security Bureau，Forensic Medical Examination and Identifican Center of Beijing Public Security Bureau，Forensic Medical Examination and Identifican Center of Beijing Public Security Bureau，Department of Forensic Medicine，Department of Forensic Medicine，Department of Forensic Medicine，Department of Forensic Medicine Medical School of Xi'an Jiaotong University，Xi'an 710061，China，Medical School of Xi'an Jiaotong University，Xi'an 710061，China，Beijing100085，China，Beijing100085，China，Beijing100085，China，Medical School of Xi'an Jiaotong University，Xi'an 710061，China，Medical School of Xi'an Jiaotong University，Xi'an 710061，China，Medical School of Xi'an Jiaotong University，Xi'an 710061，China，Medical School of Xi'an Jiaotong University，Xi'an 710061，China |
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| 基金项目： | ThisworkwassupportedbytheScientificResearchItemofXi’ancity (No .2 0 0 2 3 9) |
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| 摘 要： | CriminalratewasrisingyearafteryearinthenorthwestChina .Itisnecessarytoestablishthefo rensicDNAdatabase .Thereweremanyshorttan demrepeats (STRs)thatappearedtobeabundantandoccurred 15 - 2 0kbonaverageinthehumange nome .STRlociconsistedof 2 - 6bpinlengthasitscoresequences[1] .Thecharacteristicsofhighlyeffi cientandmultipleamplificationmadeSTRsuperiortothetraditionalgeneticmarkers .Alloftheselect edSTRlocihadcommonalleleslessthan 35 0bpinsize[2 ] .STRwasthemostidealgeneticmarkerbe causeofi…
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A NORTHWEST DATABASE MODEL OF SHORT TANDEM REPEAT LOCI IN FORENSIC MEDICINE |
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| Wang Zhenyuan,Zhu Bofeng,Liu Yacheng ,Yan Jiangwei ,Huo Zhenyi ,Jin Tianbo,Li Tao,Fan Shuanliang,Fang Jie. A NORTHWEST DATABASE MODEL OF SHORT TANDEM REPEAT LOCI IN FORENSIC MEDICINE[J]. Academic Journal of Xi’an Jiaotong University, 2003, 15(1) |
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| Authors: | Wang Zhenyuan Zhu Bofeng Liu Yacheng Yan Jiangwei Huo Zhenyi Jin Tianbo Li Tao Fan Shuanliang Fang Jie |
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| Affiliation: | 1. Department of Forensic Medicine, Medical School of Xi'an Jiaotong University, Xi'an 710061, China
2. Forensic Medical Examination and Identifican Center of Beijing Public Security Bureau, Beijing 100085, China |
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| Abstract: | Objective To establish the northwest database of short tandem repeat(STR) loci in forensic medicine. Methods Bloodstains or whole blood samples were collected from the unrelated prisoners in Xi'an city. Genetic distribution for 13 STR loci and amelogenin locus were determined in prisons based on GeneScan. One primer for each locus was labeled with the fluorescent by 5-FAM, JOE, or NED. The forensic database were generated by using multiple amplification, GeneScan, genotype, and genetic distribution analysis. Results 113 alleles and 302 genotypes were observed, with the corresponding frequency between 0.0050-0.5250 and 0.0100-0.4100. The mean H was 0.7667. The accumulative DP was 0.9999999,. The accumulative EPP was 0.9999999. The scope of PIC was 0.6036-0.8562. PM was less than 10-11. The observed and expected genotype frequencies were evaluated using χ2-test and all were in accordance with Hardy-Weinberg equilibrium (P＞0.05). Conclusion STR loci is an ideal genetic marker with powerful polymorphism and stable heredity. It can be used for individual identification and paternity in forensic medicine. The forensic DNA database model can be established successfully. |
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| Keywords: | short tandem repeat(STR) DNA database GeneScan polymerase chain reaction genotype |
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