| THE ROLE OF MUSCLE SPINDLE IN MUSCLE ATROPHY INDUCED BY SIMULATED MICROGRAVITY |
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| 作者姓名： | 张红梅 樊小力 周继斌 |
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| 作者单位： | Department of Physiology，Department of Physiology，Department of Physiology Medical School of Xi'an Jiaotong University，Xi'an 710061，China，Deparment of Physiology，Sun Yatsen University of Medical Science，Medical School of Xi'an Jiaotong University，Xi'an 710061，China，Medical School of Xi'an Jiaotong University，Xi'an 710061，China，Deparment of Physiology，Sun Yatsen University of Medical Science |
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| 基金项目： | ThisprojectwassupportedbytheNaturalScienceFoundationofShaanxiProvince,China (No.98SM5 5 ) |
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| 摘 要： | Musclesizeisdeterminedbythebalancebe tweentheratesofproteinsynthesisanddegradation .Acceleratedproteindegradationisanimportantfac torcontributingtothelossofmuscleweightinvari ousphysiologicalandpathologicalconditions .Antigravityslow twitchmusclessuchastheso leusarepreferentiallyatrophiedduringmicrogravityexperiments.Studiesbothinvivoandinvitrohaveprovidedstrongevidencethatincreased proteinbreakdownismainlyresponsibleforthesoleusmus clewastingofnon weighting bearinganimals1] ,al thoughthereis…
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THE ROLE OF MUSCLE SPINDLE IN MUSCLE ATROPHY INDUCED BY SIMULATED MICROGRAVITY |
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| Zhang Hongmei ,Fan Xiaoli,Zhou Jibin.THE ROLE OF MUSCLE SPINDLE IN MUSCLE ATROPHY INDUCED BY SIMULATED MICROGRAVITY[J].Academic Journal of Xi’an Jiaotong University,2003,15(1). |
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| Authors: | Zhang Hongmei Fan Xiaoli Zhou Jibin |
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| Institution: | 1. Deparment of Physiology,Sun Yat-sen University of Medical Science
2. Department of Physiology, Medical School of Xi'an Jiaotong University, Xi'an 710061,China |
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| Abstract: | Objective To compare the cross-section area (CSA) and the immunoreactivity of conjugated-ubiquitin in soleus extrafusal and intrafusal fibers after simulated-microgravity and to demonstrate the role of muscle spindle in muscle atrophy induced by simulated microgravity. Methods The immunohistochemical technique (ABC) and image analysis were used to assess the conjugated-ubiquitin immunostaining and the cross -sectional area of intrafusal and extrafusal fibers of soleus in simulated-microgravity rats. Results ①Tail-suspension caused a progressive loss of soleus mass. Mean fiber CSA of extrafusal fibers were (7±2)%, (21±4)% and (32±7)% smaller after 3 days, 7 days and 14 days suspension, respectively. While the CAS of intrafusal fibers (bag + chain fibers) were (14±3)% (P＜0.05), (30±7)% (P＜0.01) and (44±10)% (P＜0.01) smaller after 3 days, 7 days and 14 days suspension. ② The immunoreactivity of conjugated-ubiquitin both in extrafusal and intrafusal fibers increased after tail-suspension. The immunoreactivity of intrafusal fibers increased 1 day after suspension and reached the hightest level at 3 days after tail-suspension. The immunoreactivity of extrafusal fibers increased after 3 days suspension and reached the highest level after 7 days tail-suspension, which was lower than that in intrafusal fibers after 3 days tail-suspension. Conclusion These results suggest that soleus atrophy of intrafusal fibers caused by tail-suspension is earlier and more severe than that in extrafusal fibers. |
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| Keywords: | ubiquitin simulated microgravity muscle atrophy muscle spindle tail suspension |
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