| PRIMARY STUDY ON MECHANISM OF COLLAGEN SYNTHESIS INHIBITION IN CULTURED HUMAN FETAL HEPATOCYTES PRETREATED BY SALVIA MILTIORRHIZA BUNGE |
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| 作者姓名： | 赵英仁 朱风群 和水祥 苌新明 乔文 |
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| 作者单位： | Department of Infectious Diseases，First Hospital of Xi'an Jiaotong University，Department of Infectious Diseases，First Hospital of Xi'an Jiaotong University，Department of Internal Medicine，First Hospital of Xian Jiao tong University，Department of Internal Medicine，First Hospital of Xian Jiao tong University，Department of Internal Medicine，First Hospital of Xian Jiao tong University Xi'an 710061，China，Xi'an 710061，China，Xian 710061，China，Xian 710061，China，Xian 710061，China |
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| 摘 要： | Ithasbeenclearedthatreactiveoxygenspeciesandreactiveoxygenspecies inducedlipidperoxida tioncanstimulatecollagensynthesisofhepaticparenchymalcellsandnonparenchymalcellsandre sultinhepaticfibrosis,andthecollagensynthesisincreasingofHepatocytesmay playanimportantroleinhepaticfibrosis1 -5] .Wehavefoundthatsalviamiltiorrhizabunge (SMB)couldprotecthu manfetalhepatocytesfromcarbontetrachloride(CCl4)mediatedlipid peroxidationinjuryinvit ro6] .However,theeffectofSMBoncollagensyn thesisofhumanhepat…
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PRIMARY STUDY ON MECHANISM OF COLLAGEN SYNTHESIS INHIBITION IN CULTURED HUMAN FETAL HEPATOCYTES PRETREATED BY SALVIA MILTIORRHIZA BUNGE |
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| Zhao Yingren,Zhu Fengqun,He Shuixiang ,Chang Xinming ,Qiao Wen.PRIMARY STUDY ON MECHANISM OF COLLAGEN SYNTHESIS INHIBITION IN CULTURED HUMAN FETAL HEPATOCYTES PRETREATED BY SALVIA MILTIORRHIZA BUNGE[J].Academic Journal of Xi’an Jiaotong University,2003,15(2). |
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| Authors: | Zhao Yingren Zhu Fengqun He Shuixiang Chang Xinming Qiao Wen |
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| Institution: | 1. Department of Infectious Diseases, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China
2. Department of Internal Medicine, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China |
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| Abstract: | Objective The effects of salvia miltiorrhiza bunge (SMB) on collagen synthesis in the human fetal hepatocytes culture were studied. Methods The collagen synthesis of hepatocytes were stimulated by the addition of carbon tetrachloride (CCl 4 ) to the culture medium, the concentration of type procollagen (PC) in the culture medium and the hydroxyproline (Hyp) in hepatocytes were determined, as well as the activity of se dependent glutathione peroxidase (Se GSH Px) and the concentration of malondiadehyde (MDA) in the culture medium. Results A significant decrease in PC, Hyp and MDA production, and the significant increase in Se GSH Px activity were observed in the cultures pretreated with 1 g L -1 SMB for 4 hours compared with the untreated cultures. Analysis of the Se GSH Px/MDA ratio in SMB pretreated group showed more marked increase compared to that of the untreated group ( P <0.01). There was a significant negative correlation between the ratio of Se GSH Px/MDA and the concentration of PC in SMB pretreated group ( r=-0.9017, P <0.01). Conclusion Our results indicate that SMB may suppress the collagen synthesis of cultured human fetal hepatocytes stimulated by CCl 4 , and its mechanism may be related to the increase in Se GSH Px/MDA ratio and the enhancement of hepatocytes antioxidation capability. |
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| Keywords: | cell culture human liver collagen synthesis lipid peroxidation antioxidants salvia miltiorrhiza bunge (SMB) |
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