| 硒对体外培养人肝细胞膜流动性的影响 |
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| 引用本文： | 和水祥,舒昌杰,谢蔚玫,韩瑽,任瑛云.硒对体外培养人肝细胞膜流动性的影响[J].西安交通大学学报(医学版),1995(3). |
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| 作者姓名： | 和水祥 舒昌杰 谢蔚玫 韩瑽 任瑛云 |
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| 作者单位： | 西安医科大学第一附属医院传染科，西安医科大学第一附属医院传染科，西安医科大学第一附属医院传染科，地方性骨病研究所，地方性骨病研究所 西安710061，西安710061，西安710061 |
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| 摘 要： | 以荧光探剂(DPH)标记细胞膜脂区,利用稳态荧光偏振技术观测硒对四氯化碳损伤的原代培养人胚肝细胞膜流动性的影响,同时检测了丙二醛及硒谷胱甘肽过氧化物酶(Se-GSH-Px)活性水平。结果表明,预加硒可有效地维护受损肝细胞膜流动性。其机制可能与增强Se-GSH-Px活性,抑制脂质过氧化有关,且此作用受预作用时间、剂量等因素的影响。
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| 关 键 词： | 硒 脂质过氧化 膜流动性 培养细胞 肝细胞 |
EFFECT OF SELENIUM OF PLASMA MEMBRANE FLUIDITY OF HUMAN FETAL HEPATOCYTES IN VITRO |
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| He Shuixiang,Han Cong,Shu Changjie,et al.EFFECT OF SELENIUM OF PLASMA MEMBRANE FLUIDITY OF HUMAN FETAL HEPATOCYTES IN VITRO[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1995(3). |
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| Authors: | He Shuixiang Han Cong Shu Changjie |
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| Institution: | Department of Infectious Diseases. first Affiliated Hospital |
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| Abstract: | In order to study the effect of selenium on the of fluidity plasma membrane. human fetal hepatocytes in primary culture were pretreated with selenium and then exposed to carbon tetrachloride. The fluidity of hepatocytes plasma membrane was measured by the fluorescence polarization method using 1,6-dipheny-1,3,5-hexatriene as a fluorescentprobe. The results showed that the increase fluidity of and the activity of Se-GSH-Px, the decrease of MDA were related to the selenium concentration and protective time. It is suggested that selenium can enhance the activity of Se-GSH-Px, and inhibit the lipid peroxidation, and thus maintain the fluidity of human fetal hepatocytes plasma membrane. |
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| Keywords: | selenium lipid peroxidation membrane fluidity cultured cells human liver |
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