ICR－191体外诱发人成纤维细胞恶性转化的初步研究

ＭＳＵ－１．１细胞株是由ｖ－ｍｙｃ癌基因转染正常二倍体人成纤维细胞后所建立的生命期无限的近二倍体细胞株，使该细胞暴露于实验研究用广谱诱变剂ＩＣＲ－１９１以建立体外细胞转化体系，结果表明剂量０．３５及０．４５μｍｏｌ／Ｌ的ＩＣＲ－１９１可使ＭＳＵ－１．１细胞转化成具有转化灶形成能力和成瘤性的恶性转化细胞。本文支持永生化是人类细胞培养的多步骤的恶性转化过程的前提条件的假说。为建立的致癌物诱发细胞转化体系为研究阐明人类多步致癌的分子机理提供了一个有用的模型体系。

PRELIMINARY STUDY ON MALIGNANT TRANSFORMATION OF HUMAN FIBROBLASTS INDUCED BY ICR-191 IN VITRO

To establish a model system of cell transformation in vitro. a near-diploid, infinitive life span human fibroblast strain MSU-1.1, developed after transfection of diploid fibroblasts with v-myc oncogene, was exposed to doses of ICR-191, a versatile laboratory mutagen. The results showed that MSU-1.1 treated with ICR-191 turned into malignant cells with focus-morming ability and transplanttumorigenicity that offered further support of assumption suggesting immortalization as prerequisite in the multistep process of malignant transformation of human cell culture. This carcinogen-induced cell transformation system can be utilized as a medel systemapproaching molecular mechanisms of multistep carcinogenesis in human.