白藜芦醇治疗重症急性胰腺炎的实验研究

目的　探讨白藜芦醇对大鼠重症急性胰腺炎炎症介质的作用。方法　应用逆行胰胆管穿刺注射牛黄胆酸钠制备重症急性胰腺炎大鼠模型。90 只大鼠随机分为假手术组、重症急性胰腺炎模型组(SAP)、白藜芦醇治疗组I(RES1,5mg/kg)、白藜芦醇治疗组II(RES2,10mg/kg)、白藜芦醇治疗组III(RES3,20mg/kg)等5 组,每组18 只。术后3、6、12h各剖杀6只,观察重症急性胰腺炎大鼠模型腹水的性质、量,测定血清肿瘤坏死因子、白细胞介素6 的水平,观察治疗组和模型组生存率的变化。结果　白藜芦醇治疗组与重症急性胰腺炎模型组相比,血清中肿瘤坏死因子、白细胞介素6的水平明显下降,死亡率降低,腹水量减少(P<0.05)。结论　白藜芦醇降低重症急性胰腺炎大鼠死亡率,减少腹水量可能与抑制血清肿瘤坏死因子及白细胞介素6的水平有关。

Experimental study of resveratrol on severe acute pancreatitis

Objective To investigate the effect of resveratrol on cytokines in rat models with severe acute pancreatitis (SAP). Methods SAP model was made by injecting taurocholic acid 0.1 mL/100g to pancreatic bile duct. Animals were divided into five groups: control group, SAP model group and resveratrol treated group Ⅰ(RES1, 5 mg/kg), Ⅱ(RES2, 10 mg/kg)and Ⅲ(RES3, 20 mg/kg). Animals were sacrificed at 3 h, 6 h and 12 h after inducing SAP model. The quality and quantity of ascites, and survival rate were observed. The levels of serum IL-6 and TNF were measured by radioimmunoassay. Results Resveratrol decreased the levels of serum IL-6 and TNF significantly and reduced ascitic fluid and the mortality rate (P<0.05). Conclusion The reduced ascitic fluid and the mortality rate by resveratrol in taurocholic acid-induced SAP model might be related to its inhibiting the release and activation of TNF and IL-6.