自体淋巴细胞免疫重建对白血病瘤苗的加强作用

目的观察在免疫重建过程中应用白血病瘤苗对DBA/2小鼠的一般状况、抵御L1210细胞攻击以及小鼠保护性免疫功能的影响。方法用同系小鼠脾脏淋巴细胞对照射后的免疫缺陷鼠进行免疫重建,同时用灭活的L1210细胞皮下注射免疫DBA/2小鼠,7d后用L1210细胞皮下攻击小鼠,观察小鼠的一般状况、成瘤率、瘤块生长情况。通过ELISA法测定外周血IFN-γ含量,以评估细胞免疫作用的强弱;通过病理切片了解瘤块中心坏死区及周围浸润细胞情况。结果与对照组相比,免疫重建+瘤苗可以有效提高单纯应用瘤苗所产生的保护性免疫功能,IFN-γ含量明显升高;用L1210细胞攻击,成瘤小鼠瘤块生长缓慢,瘤周单个核细胞浸润明显,瘤块内部肿瘤细胞坏死明显,生存期大大延长。结论在免疫重建的同时应用瘤苗,可以大大加强瘤苗的保护性免疫作用。

Enhanced protective immunity in DBA/2 mice that receive sublethal irradiation and immune reconstitution

Objective To observe the general status and the immunity of DBA/2 mice injected with leukemia vaccine during the immune reconstitution, and to observe the survival rate of these injected mice that were then challenged by L1210 cells. Methods Normal mice and reconstituted lymphopenic mice (RLM; DBA/2 mice rendered lymphopenic with sublethal irradiation and reconstituted with naive splenocytes) were used in the vaccination and challenge experiments with L1210 cells. Results Following vaccination, a significant increase of the IFN-γ was detected in the blood of vaccinated RLM compared with that of vaccinated normal mice, as assessed by ELISA. Significantly, greater protection was induced in vaccinated RLM, which led to a stronger protection from death. Conclusion The vaccination of reconstituted lymphopenic hosts could elicit superior anti-tumor immunity compared with normal hosts, highlighting the potential clinical benefit of performing tumor vaccination during immune reconstitution.