| MITOCHONDRIAL DNA POLYMORPHISM IN CONTROL REGION FROM CHINESE YUGU POPULATION |
| |
| 作者姓名： | 刘新社 李生斌 |
| |
| 作者单位： | [1]NationalKeyLaboratoryofForensicSciences,Xi＇anJiaotongUniversity,Xi＇an710061,China [2]NationalKeyLaboratoryofForensicSciences,Xi＇anJiaotongUniversity,Xi＇an710061,China |
| |
| 基金项目： | ThisstudywassupportedbytheNationalKeyTechnologiesR&DPrograminthe9th“fiveyearplan”ofChina(No.9691901043). |
| |
| 摘 要： | Objective To investigate the mitochondrial DNA sequence polymorphism sites in Chinese YUGU ethnic group and to provide basic data used in forensic purpose. Methods Genomic DNA was extracted from the hole blood of 100 unrelated individuals of Chinese YUGU ethnic group by standard chelex-100 method. The sequence polymorphism sites was determined by PCR amplification and direct sequencing. Results 54 polymorphic sites were noted in mtDNA np16091-16418 region, and 46 haplotypes were identified. The genetic diversity was calculated to be 0. 9691, and the genetic identity was calculated to be 0. 0406. Conclusion There are some particular polymorphism sites in Chinese YUGU ethnic group. The results suggest that sequence polymorphism from np]6091 -- 16418 in human mitochondrial DNA can be used as a biological marker for forensic identity.
|
| 关 键 词： | DNA 多态现象 控制区 HVSⅠ 种族 |
MITOCHONDRIAL DNA POLYMORPHISM IN CONTROL REGION FROM CHINESE YUGU POPULATION |
| |
| Liu Xinshe,Li Shengbin National Key Laboratory of Forensic Sciences,Xi'an Jiaotong University,Xi'an ,China..MITOCHONDRIAL DNA POLYMORPHISM IN CONTROL REGION FROM CHINESE YUGU POPULATION[J].Academic Journal of Xi’an Jiaotong University,2004,16(2):174-177. |
| |
| Authors: | Liu Xinshe Li Shengbin National Key Laboratory of Forensic Sciences Xi'an Jiaotong University Xi'an China |
| |
| Institution: | National Key Laboratory of Forensic Sciences, Xi'an Jiaotong University, Xi'an 710061, China. |
| |
| Abstract: | Objective To investigate the mitochondrial DN A sequence polymorphism sites in Chinese YUGU ethnic group and to provide basic da ta used in forensic purpose. Methods Genomic DNA was extracted from the hole blood of 100 unrelated individuals of Chinese YUGU ethnic group by standard chelex-100 method. The sequence polymorphism sites was determined by PCR amplification and direct sequencing. Results 54 polymorphic sites were noted in mtDNA np16091-16418 region, and 46 haplotypes were identifi ed. The genetic diversity was calculated to be 0.9691, and the genetic identity was calculated to be 0.0406. Conclusion There are some particul ar polymorphism sites in Chinese YUGU ethnic group. The results suggest that seq uence polymorphism from np16091-16418 in human mitochondrial DNA can be used as a biological marker for forensic identity. |
| |
| Keywords: | mtDNA polymorphism control region HVSⅠ |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
