SP和Con A对培养的大鼠皮肤肥大细胞组胺释放的影响

用酶消化法获得大鼠皮肤肥大细胞，培养于含10％胎牛血清的DMEM培养液。培养1d后，在培养液中加入不同浓度的SP、SP＋Ca2+或ConA，继续培养不同时间。用微孔滤膜迅速分离细胞和培养液，荧光法测定培养液中的组胺含量，计算组胺释放率。结果显示，SP和ConA均能以时间和剂量依赖性方式刺激体外培养的大鼠皮肤肥大细胞释放组胺，并且SP的这种作用明显地受Ca2+浓度的影响。

INFLUENCE OF SP AND CON A ON HISTAMINE RELEASE FROM CULTURED MAST CELLS OF RAT SKIN

The mast cells from enzymatically dispersed rat skin were incubated in DMEM medium containing 10% fetal bovine serum. I day after culture, the various concentrations of substance P (SP), SP Ca2 or concanavalin A (Con A) were added into the media to stimulate mast cells for different periods of time. The media were rapidly seperated from the cultured cells using the micropore filter. The contents of histamine in the media were determined by spectronuorimetry and the release rates of histamine were caculated. The results showed that SP and Con A could stimulate in petromast cells to release histamine in a time--and dosedependent pattern, and that the effect of SP was significantly influenced ac the concentration of Ca2 .