非小细胞肺癌患者血清及癌组织中P185neu的定量检测

目的探讨P185neu蛋白的定量水平与非小细胞肺癌(NSCLC)患者的临床生物学行为及预后的关系。方法采用双抗体夹心ELISA法定量检测了37例NSCLC患者及17例良性肺疾病和健康对照者血清和组织中P185neu的蛋白水平。结果37例NSCLC患者血清和癌组织中P185neu的蛋白水平明显升高(P<0.01),且P185neu蛋白水平与NSCLC细胞分化程度、TNM分期及淋巴结转移呈显著正相关(P<0.05)。另外,NSCLC患者术前血清P185neu的蛋白水平明显高于术后10天的血清水平(P<0.01)。结论P185neu的蛋白水平与NSCLC的发生、发展和转移密切相关;血清P185neu蛋白水平可作为评估NSCLC进展、复发和预后的有效指标。

Determination of P185neu level in serum and tumour tissue of non-small cell lung cancer

Objective To investigate the relation between the level of P185neu in serum and tumour tissue and clinical biological behaviour of non-small cell lung cancer(NSCLC).Methods By ELISA method,37 specimens of sera and tumour tissues of non-small cell lung cancer were examined,and 17 cases of adjacent nonneoplastic tissues and sera were examined for the level of P185neu protein.Results Of the 37 NSCLC cases,the level of P185neu in sera and tumour tissues was higher(P<0.01).The level of P185neu was positively related to cell differentiation,TNM stages,and lymph node metastasis(P<0.05).The preoperative serum level of P185neu was significantly higher than the postoperative serum level(P<0.01).Conclusion P185neu may play an important role in tumor progression and malignant potential of non-small cell lung cancer,and the level of serum P185neu could be of prognostic significance.