EFFECTS OF FILTER MEMBRANES ADSORBING INFLAMMATORY MEDIATORS IN HEMOFILTRATION

Sepsisischaracterizedbyreleaseofexcessiveamountofproinflammatorycytokinesinthecircu lation ,leadingtoa generalizedanduncontrolledhostresponse .Aconsiderablenumberofexperimen talandclinicalinvestigatorshavereportedemploy inghemofiltration (HF)toeliminatecytokinesandchemokines .Thisexcessiveinflammatoryresponsecouldbedownregulatedwithremovalofcytokinesandothermediators .Thesedatashowedthatthemechanismofmediatorseliminationwaslinkedtoconvectionandadsorptionbyhemofiltration .Ad sorptionappearstob…

EFFECTS OF FILTER MEMBRANES ADSORBING INFLAMMATORY MEDIATORS IN HEMOFILTRATION

Objective In this study, an in vitro hemofiltration model was set up to investigate adsorptive saturation time of different membrane under different blood flow rate (QB)and filtrate rate(QF). Methods Anticoagulated cattle blood (2000mL per bag) was stimulated with 1μg*mL-1 endotoxin to induce inflammatory mediators before hemofiltration (HF) using AN69, PS and PMMA filters in vitro. Adsorptive saturation time of membrane was observed using data bridge in different QB and different QF. TNF was measured by radioimmunoassays. Results Before the resistance level reached the peak value in the same group, resistance level increased significantly (P<0.01). After the peak value, there was no difference (P>0.05). It was suggested that the resistance level reached plateau at 150,120,90,120, and150 minutes in QF of 100mL*minute-1, 200mL*minute-1, 300mL*minute-1, respectively and in QF of 1L*hour-1, 2L*hour-1, 4L*hour-1, respectively. And with the QB and QF increasing, resistance level increased significantly (P<0.01) among different groups at the same time point in A, B, C, D and E group. Conclusion Membrane resistance level online measured by Data Bridge can instantly reflect the degree of membrane adsorption. Adsorptive saturation time of filter membrane in different filtration flow rate and blood flow rate are different.