| 乌头碱在家兔检材中的降解动力学 |
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| 引用本文： | 邓新生,胡炳蔚.乌头碱在家兔检材中的降解动力学[J].西安交通大学学报(医学版),1990(1). |
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| 作者姓名： | 邓新生 胡炳蔚 |
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| 作者单位： | 西安医科大学法医系
(邓新生)，西安医科大学法医系(胡炳蔚) |
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| 摘 要： | 用高效液相色谱法(High performance liquid chromatography,HPLC)分离测定生物性样品中的乌头碱。对乌头碱在离体生物检材中的降解动力学进行了研究。乌头碱在经不同处理的生物检材(凝血块、肝脏)中以表观二级动力学过程被降解,其降解速率常数K以4%甲醛溶液固定组中为最大(1.5619,0.7728μg/d),其次为95%酒精固定组(0.4891,0.7556μg/d),4℃冰箱放置组中最小(0.06372,0.0393μg/d),亦即中毒检材中乌头碱在冰箱中分解较慢,其次为在酒精固定剂中。从死亡或中毒后一段时间取检材检测,可用建立的动力学方程推算体內乌头碱是否已达中毒量或致死量。
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| 关 键 词： | 高效液相色谱法 乌头碱 降解动力学 检材保存 死亡时间 |
DEGRADATION KINETICS OF ACONITINE IN THE SAMPLE OF RABBITS |
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| Deng Xinsheng Hu Bingwei.DEGRADATION KINETICS OF ACONITINE IN THE SAMPLE OF RABBITS[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1990(1). |
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| Authors: | Deng Xinsheng Hu Bingwei |
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| Institution: | Faculty of Forensic Medicine |
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| Abstract: | A high performance liquid chromatography (HPLC)has been used to determine aconitine in the sample of rabbits. Kinetics of degradation of aconitine in vitro was studied. The degradation of aconite from the poisoned specimens (coagulated blood, liver) treated differently in vitro was characterized by pseudo-secondorder kinetics. The K. of aconitine in these samples was the biggest in the fixed section of 4% aqueous solution of formaldehyde (1.5619, O.7728μg/d), bigger in the fixed section of 95% alcohol(0.4891, 0.07536μg/d) and the smallest in the refrigerator at 4℃ (0.06372, 0.03903 μg/d), respectively. That is, aconitine in the poisoned samples was decomposed slowly in the refrigerator and secondly in the fixative of alcohol. Amount of aconitine might be extrapolated from the result of examination of specimens taken from the subject after poisoning or killing by the poison, using the kinetics equation. |
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| Keywords: | high performance liquit chromatography aconitine degradation kinetics sample preserve time since death |
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