恶性梗阻性黄疸实验模型建立及评价

目的　建立SD大鼠肝癌侵袭胆管所致恶性梗阻性黄疸模型 ,初步评价其临床应用价值。方法　用Walker- 2 5 6瘤株近肝门部肝实质内接种 ,致高位胆管浸润转移造成狭窄 ,引起梗阻性黄疸。结果　本实验模型所致梗阻性黄疸表现及肝功能变化为慢性渐进性发展过程 ,病理切片光镜检查显示肝脏、胆管有癌细胞浸润和转移 ,同时肝内有不同程度的胆汁淤积表现。结论　此实验模型稳定、可重复 ,成功率为 73 .3 % ,其梗阻性黄疸表现特点与临床恶性梗阻性黄疸病人症状极其相似 ,对研究恶性梗阻性黄疸的病理生理机制及治疗具有一定价值。

The establishment and evaluation of malignant biliary obstruction mod el on the SD rats

Objective To establish malignant biliary obstruction model on the SD rats and to discuss its value and effect in the clinical application. Methods The model of malignant obstructive jaundice was made by vaccination of transplanted tumor in liver nearby the porta hepatis on SD rats with the Walker-256 hepatocarcinoma cell line, which infiltrated to the common bile duct and led to high biliary obstruction.Results The process of the obstructive jaundice developed chronically and gradually in clinical expression and liver function. Pathologically, the metastasis and infiltration of carcinocytes were found in common bile duct with cholestasis.Conclusion The experimental model on SD rats is stable and reproductive. Its successful rate is 73.3%.The clinical manifestation of this model is similar to that of patients with malignant biliary obstruction. This model might be valuable of researching the mechanism of the malignant obstructive jaundice.