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CPG OLIGONUCLEOTIDES REGULATE OSTEOCLAST DIFFERENTIATION
作者姓名:赵为公  韩学哲  李新友  郭雄  刘淼
作者单位:Orthopaedic Department,First Hospital of Xi'an Jiaotong University,Xi'an 710061,China,Orthopaedic Department,First Hospital of Xi'an Jiaotong University,Xi'an 710061,China,Orthopaedic Department,First Hospital of Xi'an Jiaotong University,Xi'an 710061,China,Orthopaedic Department,First Hospital of Xi'an Jiaotong University,Xi'an 710061,China,Orthopaedic Department,First Hospital of Xi'an Jiaotong University,Xi'an 710061,China
摘    要:

关 键 词:M-CSF  发炎  骨髓单核细胞  寡核苷酸

CPG OLIGONUCLEOTIDES REGULATE OSTEOCLAST DIFFERENTIATION
Zhao Weigong,Han Xuezhe,Li Xinyou,Guo Xong,Liu Miao.CPG OLIGONUCLEOTIDES REGULATE OSTEOCLAST DIFFERENTIATION[J].Academic Journal of Xi’an Jiaotong University,2005,17(1):90-93,96.
Authors:Zhao Weigong  Han Xuezhe  Li Xinyou  Guo Xong  Liu Miao
Institution:Orthopaedic Department, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China
Abstract:Objective Bacterial DNA is a pathogen-derived molecule which can regulate the innate immune system by stimulating NF-κB activation. The activity of bacterial DNA relies on its content of unmethylated CpG dinucleotides in particular base contexts("CpG motif"). In light of the pivotal role played by NF-κB in osteoclast differentiation, the ability of CpG oligodeoxynucleotides (CpG ODN) coming from bacterial DNA to modulate osteoclastogenesis was studied. Methods Bone marrow mononuclear cells (BMM) were purified from Balb/c mice, cultured in α-MEM media containing 10% FCS in the presence of mouse M-CSF, with either RANKL or ODNs for 5 days. Osteoclast formation was evaluated on day 5 according to TRAP and May-Grunwald-Giemsa staining. Results CpG ODN alone could induce osteoclast formation in the low degree in BMM culture. The relationship between CpG ODN and RANKL was that CpG ODN could inhibit RANKL-induced osteoclastogenesis when present from the beginning of BMM culture, but strongly increased RANKL-induced osteoclastogenesis in RANKL-pretreated BMMs. Conclusion The mechanism of CpG ODN regulating osteoclast differentiation was bidirectional, which might be a potential therapy for treating metabolic bone disease.
Keywords:RANKL  M-CSF  bone marrow mononuclear cell  oligodexynucleotide
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