| 尖锐湿疣中人乳头瘤病毒DNA检测及存在情况初探 |
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| 引用本文： | 董小平,袁育康,房益兰,刘延娜,楚雍烈,任会勋.尖锐湿疣中人乳头瘤病毒DNA检测及存在情况初探[J].西安交通大学学报(医学版),1992(2). |
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| 作者姓名： | 董小平 袁育康 房益兰 刘延娜 楚雍烈 任会勋 |
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| 作者单位： | 西安医科大学微生物学教研室，西安医科大学微生物学教研室，西安医科大学微生物学教研室，西安医科大学微生物学教研室，西安医科大学微生物学教研室，西安医科大学微生物学教研室 西安 710061，西安 710061，西安 710061，西安 710061，西安 710061，西安 710061 |
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| 摘 要： | 尖锐湿疣的发病与人乳头瘤病毒密切相关。本文用打点杂交法检测了28例尖锐湿疣病理标本,其中14例与HPV-11探针杂交阳性,阳性率50%(14/28)。对其中杂交信号较强的9例进一步分别用BamI Ⅱ、PstI酶解,Southem转印后与HPV-11探针杂交,对病毒DNA存在形式进行分析,结果证实HPV-11在尖锐湿疣细胞中主要以游离形式存在。
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| 关 键 词： | 人乳头瘤病毒 尖锐湿疣 打点杂交 Southern特印 |
DETECTION AND ANALYSIS OF HUMAN PAPILLOMAVIRUS DNA IN CONDYLOMA ACUMINATUM |
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| Dong Xiaoping,Yuan Yukang,Fang Yilan,et al.DETECTION AND ANALYSIS OF HUMAN PAPILLOMAVIRUS DNA IN CONDYLOMA ACUMINATUM[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1992(2). |
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| Authors: | Dong Xiaoping Yuan Yukang Fang Yilan |
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| Institution: | Department of Microbiology |
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| Abstract: | Condyloma acuminatum is closely associated with human papillomavirus (HPV). In this study the method of slotting hybridization was used to detect the Specimens of 28 cases of condyloma acuminatum. Fourteen out of the 28 samples were positive for HPV 11 probe, the positive rate was 50% (14/28). Nine out of the 14 positive samples were digested with Bamll Ⅰ and Pst Ⅰ respectively to study the present state of DNA after using Southern blotting hybridization. It was shown that HPV 11 DNA were mainly present in a simple monometric episomal state in the condyloma acuminatum cells. |
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| Keywords: | Condyloma acuminatum I luman papillomavirus Slot hybridization Southern blot |
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