| 用薄层色谱法测定骨康片中士的宁和马钱子碱含量 |
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| 引用本文： | 牛晓峰,李维凤,歧林,李延.用薄层色谱法测定骨康片中士的宁和马钱子碱含量[J].西安交通大学学报(医学版),2000,21(5):476-479. |
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| 作者姓名： | 牛晓峰 李维凤 歧林 李延 |
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| 作者单位： | 西安交通大学药学院，西安 710061 |
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| 摘 要： | 目的 建立骨康片中士的宁和马钱子碱的含量测定方法。方法 采用双波长薄层扫描法，硅胶GF２５４薄层板，展开剂为甲苯丙酮乙醇浓氨试液(4∶3∶1∶2)，检测波长，士的宁λＳ＝２５８nｍ，λＲ＝３１０ｎｍ；马钱子碱λＳ＝３０１ｎｍ，λＲ＝３３５ｎｍ。结果 士的宁和马钱子碱的相关系数分别为0..9996和0..9999，方法的回收率分别为99..83%(RSD=1.03%)和96..09%(RSD=2.01%)。结论 方法准确、简便、重现性好，同时可以定量测定骨康片中士的宁和马钱子碱含量，可作为该药的质量控制方法。
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| 关 键 词： | 骨康片 士的宁 马钱子碱 双波长薄层扫描法 |
| 文章编号： | 0258-0659(2000)05-0476-02 |
| 修稿时间： | 2000年1月1日 |
Simultaneous determination of strychnine and brucine in Gukang tablet by TLC |
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| Niu Xiaofeng.Simultaneous determination of strychnine and brucine in Gukang tablet by TLC[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2000,21(5):476-479. |
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| Authors: | Niu Xiaofeng |
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| Abstract: | Objective To establish the analytical method of strychnine and brucine in Gukang tablet by TLC. Methods Strychnine and brucine in the tablet were separated by TLC using silica stationary phase and toluene/acetone/alcohol/amine solution (4:3:1:2) as developing solution. The detection was at 258nm for strychnine, 301nm brucine. Results The linearities were 0. 9996 and 0. 9999, recoveries were 99.83 % (RSD=1.03%) and 96.09 % (RSD=2.01％), respectively. Conclusion The strychnine and brucine in Gukang tablet were simultaneously detected by TLC. The method can use for controlling quality of the tablet. |
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| Keywords: | Gukang tablet strychnine brucine TLC |
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