| 免疫酶联吸附试验检测流行性出血热抗体的初步报道 |
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| 引用本文： | 岐天茂,吴文琪,姜文榕,曹振平,谢蔚玫. 免疫酶联吸附试验检测流行性出血热抗体的初步报道[J]. 西安交通大学学报(医学版), 1986, 0(1) |
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| 作者姓名： | 岐天茂 吴文琪 姜文榕 曹振平 谢蔚玫 |
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| 作者单位： | 西安医科大学病毒学研究室(岐天茂，吴文琪，姜文榕，曹振平)，西安医科大学第一附属医院传染病学教研室(谢蔚玫) |
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| 摘 要： | 本文着重介绍ELISA检测出血热患者血清IgG抗体方法,并与IFAT进行比较,两方法阳性符合率及阴性符合率是令人满意的,特别是EHF患者发热初期ELISA抗体阳性检出率明显高于IFAT法。实验发现,临床重危型患者发热期ELISA抗体滴度多数高于IFAT抗体滴度2～4倍,而恢复期则正好相反。故该方法适用于EHF临床诊断及流行病学调查。因ELISA系借助酶标检测仪判读结果。数据准确,无检测仪器者可以目测此色,判断结果,故便于基层人员推广使用。
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DETECTION OF ANTIBODY TO EPIDEMIC HEMORRHAGIC FEVER BY ELISA METHOD |
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| Qi Tianmao,et al. DETECTION OF ANTIBODY TO EPIDEMIC HEMORRHAGIC FEVER BY ELISA METHOD[J]. Journal of Xi‘an Jiaotong University:Medical Sciences, 1986, 0(1) |
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| Authors: | Qi Tianmao et al |
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| Affiliation: | Research Laboratoty of Virology |
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| Abstract: | This paper reports the method for detecting the antibodies to epidemic hemorrhagic fever (EHF), compared with the method of IFA. The results were satisfactory, We found that the antibody positive rate detected by enzyme-linked immunosorbent assay in the patients with early EHF was significantly higher than that by IFA. It was noticed that the antibody titer of those patients who were in the early stage of fever was twice to four times higher than that by IFA. The method reported here might be a useful technique for clinical diagnosis and epidemiological reseach and for the country doctors. |
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| Keywords: | antibodies enzyme-Linked immunosorbent assay fluorescent antibody technic |
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